Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/90053
Title: Thermal acclimation and seasonal acclimatization: A comparative study of cardiac response to prolonged temperature change in shorthorn sculpin
Authors: Filatova, T. S.
Abramochkin, D. V.
Shiels, H. A.
Issue Date: 2019
Publisher: Company of Biologists Ltd
Citation: Filatova, T. S. Thermal acclimation and seasonal acclimatization: A comparative study of cardiac response to prolonged temperature change in shorthorn sculpin / T. S. Filatova, D. V. Abramochkin, H. A. Shiels. — DOI 10.1242/jeb.202242 // Journal of Experimental Biology. — 2019. — Vol. 16. — Iss. 222. — jeb202242.
Abstract: Seasonal thermal remodelling (acclimatization) and laboratory thermal remodelling (acclimation) can induce different physiological changes in ectothermic animals. As global temperatures are changing at an increasing rate, there is urgency to understand the compensatory abilities of key organs such as the heart to adjust under natural conditions. Thus, the aim of the present study was to directly compare the acclimatization and acclimatory response within a single eurythermal fish species, the European shorthorn sculpin (Myoxocephalus scorpio). We used current- and voltage-clamp to measure ionic current densities in both isolated atrial and ventricular myocytes from three groups of fish: (1) summer-caught fish kept at 12°C (‘summer-acclimated’); (2) summer-caught fish kept at 3°C (‘cold acclimated’); and (3) fish caught in March (‘winter-acclimatized’). At a common test temperature of 7.5°C, action potential (AP) was shortened by both winter acclimatization and cold acclimation compared with summer acclimation; however, winter acclimatization caused a greater shortening than did cold acclimation. Shortening of AP was achieved mostly by a significant increase in repolarizing current density (IKr and IK1) following winter acclimatization, with cold acclimation having only minor effects. Compared with summer acclimation, the depolarizing L-type calcium current (ICa) was larger following winter acclimatization, but again, there was no effect of cold acclimation on ICa. Interestingly, the other depolarizing current, INa, was downregulated at low temperatures. Our further analysis shows that ionic current remodelling is primarily due to changes in ion channel density rather than current kinetics. In summary, acclimatization profoundly modified the electrical activity of the sculpin heart while acclimation to the same temperature for >1.5 months produced very limited remodelling effects. © 2019. Published by The Company of Biologists Ltd.
Keywords: ACTION POTENTIAL
ELECTROPHYSIOLOGY
HEART
HYPERTROPHY
MYOXOCEPHALUS SCORPIO
THERMAL REMODELLING
ACCLIMATIZATION
ACTION POTENTIAL
ANIMAL
CARDIAC MUSCLE CELL
FISH
HEAT
HEAT TOLERANCE
PHYSIOLOGY
SEASON
ACCLIMATIZATION
ACTION POTENTIALS
ANIMALS
FISHES
HOT TEMPERATURE
MYOCYTES, CARDIAC
SEASONS
THERMOTOLERANCE
URI: http://hdl.handle.net/10995/90053
https://elar.urfu.ru/handle/10995/90053
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85071707207
WOS ID: 000484368200004
PURE ID: 10768470
ISSN: 0022-0949
DOI: 10.1242/jeb.202242
metadata.dc.description.sponsorship: Russian Foundation for Basic Research, RFBR: 18-315-20049
The study was supported by the Russian Foundation for Basic Research (18-315-20049 to D.V.A.).
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

Files in This Item:
File Description SizeFormat 
10.1242-jeb.202242.pdf1,8 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.