Compartmentation of Photosynthesis in Cells and Tissues of C4 Plants

Abstract

Critical to defíning photosynthesis in C4 plants is understandíng the intercellular and intracellular compartmentation of enzymes between mesophyll and bundle sheath cells ín the leaf. Thís íncludes enzymes of the C4 cycle (includíng three subtypes), the C3 pathway and photorespiration. The current state of knowledge of this compartmentatíon is a consequence of the development and applicatíon of different techniques over the past three decades. Initial studies led to some alternative hypotheses on the mechanism of C4 photosynthesis, and some controversy over the compartmentation of enzymes. The development of methods for separating mesophyll and bundle sheath cells provided convincing evidence on intercellular compartmentation of the key components of the C4 pathway. Studies on the intracellular compartmentation of enzymes between organelles and the cytosol were facilitated by the isolation of mesophyll and bundle sheath protoplasts, which can be fractionated gently while maintaining organelle integrity. Now, the ability to determine localization of photosynthetic enzymes conclusively, through in situ immunolocalization by confocal light microscopy and transmission electron microscopy, is providing further insight into the mechanism of C4 photosynthesis and its evolution. Currently, immunological, ultrastructural and cytochemical studies are revealing relationships between anatomícal arrangements and photosynthetic mechanísms which are probably related to environmental factors assocíated wíth evolutíon of these plants. This íncludes interesting variations in the C4 Syndrome in leaves and cotyledons of species ín the tríbe Salsoleae of the famíly Chenopodiaceae, in relatíon to evolutíon and ecology. Thus, analysis of structure-functíon relatíonships using modern techniques is a very powerful approach to understanding evolution and regulation of the photosynthetic carbon reduction mechanisms.