Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/111877
Title: Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum
Authors: Khokhlova, A.
Myachina, T.
Volzhaninov, D.
Butova, X.
Kochurova, A.
Berg, V.
Gette, I.
Moroz, G.
Klinova, S.
Minigalieva, I.
Solovyova, O.
Danilova, I.
Sokolova, K.
Kopylova, G.
Shchepkin, D.
Issue Date: 2022
Publisher: MDPI
MDPI AG
Citation: Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum / A. Khokhlova, T. Myachina, D. Volzhaninov et al. // International Journal of Molecular Sciences. — 2022. — Vol. 23. — Iss. 3. — 1719.
Abstract: Type 1 diabetes (T1D) leads to ischemic heart disease and diabetic cardiomyopathy. We tested the hypothesis that T1D differently affects the contractile function of the left and right ventricular free walls (LV, RV) and the interventricular septum (IS) using a rat model of alloxan-induced T1D. Single-myocyte mechanics and cytosolic Ca2+ concentration transients were studied on cardiomyocytes (CM) from LV, RV, and IS in the absence and presence of mechanical load. In addition, we analyzed the phosphorylation level of sarcomeric proteins and the characteristics of the actin-myosin interaction. T1D similarly affected the characteristics of actin-myosin interaction in all studied regions, decreasing the sliding velocity of native thin filaments over myosin in an in vitro motility assay and its Ca2+ sensitivity. A decrease in the thin-filament velocity was associated with increased expression of β-myosin heavy-chain isoform. However, changes in the mechanical function of single ventricular CM induced by T1D were different. T1D depressed the contractility of CM from LV and RV; it decreased the auxotonic tension amplitude and the slope of the active tension-length relationship. Nevertheless, the contractile function of CM from IS was principally preserved. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords: ACTIN-MYOSIN INTERACTION
CALCIUM TRANSIENTS
DIABETIC CARDIOMYOPATHY
INTERVENTRICULAR DIFFERENCES
SINGLE CARDIOMYOCYTE MECHANICS
TENSION-LENGTH RELATIONSHIP
CALCIUM
ANIMAL
CARDIAC MUSCLE CELL
HEART CONTRACTION
HEART VENTRICLE FUNCTION
INSULIN DEPENDENT DIABETES MELLITUS
MALE
METABOLISM
PATHOLOGY
RAT
WISTAR RAT
ANIMALS
CALCIUM
DIABETES MELLITUS, TYPE 1
MYOCARDIAL CONTRACTION
MYOCYTES, CARDIAC
RATS
RATS, WISTAR
VENTRICULAR FUNCTION
URI: http://hdl.handle.net/10995/111877
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85124365907
PURE ID: 29632037
ISSN: 1661-6596
metadata.dc.description.sponsorship: Funding: This work was supported by the Russian Science Foundation № 18-74-10059. The work was performed using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology.
RSCF project card: 18-74-10059
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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