Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/111179
Title: Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
Authors: Kulikova, T.
Porfireva, A.
Rogov, A.
Evtugyn, G.
Issue Date: 2021
Publisher: MDPI
MDPI AG
Citation: Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode / T. Kulikova, A. Porfireva, A. Rogov et al. // Sensors. — 2021. — Vol. 21. — Iss. 22. — 7763.
Abstract: Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords: CARBON BLACK
CYCLIC VOLTAMMETRY
DOXORUBICIN DETERMINATION
DRUG DETERMINATION
ELECTROCHEMICAL DNA SENSOR
CARBON BLACK
CHEMICAL DETECTION
CYCLIC VOLTAMMETRY
DIMETHYLFORMAMIDE
GLASS
GLASS MEMBRANE ELECTRODES
SULFUR COMPOUNDS
ANTITUMOR DRUGS
DNA INTERACTION
DNA SENSORS
DOXORUBICIN
DOXORUBICIN DETERMINATION
DRUG DETERMINATION
ELECTROCHEMICAL DNA SENSOR
GLASSY CARBON ELECTRODES
IRREVERSIBLE OXIDATION
SENSITIVE DETECTION
DNA
ACRIDINE YELLOW
AMINOACRIDINE DERIVATIVE
CARBON
DNA
ELECTROCHEMICAL ANALYSIS
ELECTRODE
AMINOACRIDINES
CARBON
DNA
ELECTROCHEMICAL TECHNIQUES
ELECTRODES
URI: http://hdl.handle.net/10995/111179
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85119334257
PURE ID: 28943188
ISSN: 1424-8220
metadata.dc.description.sponsorship: This research was funded by the Russian Science Foundation (A.P., grant no. 17-73-20024, investigations of DNA adsorption DMF influence and DNA-doxorubicin interactions) and the Russian Foundation for Basic Research (G.E., grant no. 20-03-00207, investigation of the AY electrochemical properties and its transfer on the electrode interface).
RSCF project card: 17-73-20024
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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