Влияние глиального нейротрофического фактора (GDNF) на уровень мРНК генов апоптоза Bax и Bcl-xl в мозге мышей с генетической предрасположенностью к патологическому поведению

Abstract

We investigated the effects of GDNF central administration on mRNA level of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-xl in brain of mice ASC strain, genetically predisposed to depressive-like behavior, and parental “nondepressive” CBA strain. It was found that GDNF injection lead to increase of Bcl-xl mRNA level in hippocampus of mice both strains, and also Bax mRNA level in hippocampus of ASC mice. Moreover, we found significant interstrain differences in Bax and Bcl-xl mRNA levels. The Bax mRNA level was increased in all investigated brain structures of ASC mice, and Bcl-xl mRNA level was increased in midbrain. Thus, we showed anti-apoptotic effect of GDNF in vivo. Furthermore, the results indicate both activation apoptosis processes in brain ASC mice and significant compensatory changes probably directed on elevation of the threshold for neuronal apoptosis.