Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/103319
Title: Method development and validation of a reversed phase HPLC method for determination of Anastrazole and Temozolomide in pharmaceutical dosage form
Authors: Akram, N. M. D.
Gopal, N. M.
Balakrishna, A.
Reddy, N. B.
Issue Date: 2020
Publisher: American Institute of Physics Inc.
Citation: Method development and validation of a reversed phase HPLC method for determination of Anastrazole and Temozolomide in pharmaceutical dosage form / N. M. D. Akram, N. M. Gopal, A. Balakrishna, et al. — DOI 10.1063/5.0018206 // AIP Conference Proceedings. — 2020. — Vol. 2280. — 0018206.
Abstract: A new simple assay method has been developed and validated for the determination of Anastrazole and Temozolomide using reverse-phase high-performance liquid chromatography in their pharmaceutical dosage form. The chromatographic separation was performed on an Inertsil ODS (4.6 x 150mm, 5m) using mobile phase phosphate buffer pH 3.0 and methanol of 30:70% v/v at a flow rate of 0.8mL/min. Analytes were detected at 260nm. The method was found to be linear in the concentration range of 1-5μg/mL for both medicaments with the coefficient value (R2) of >0.999. The accuracy was measured via recovery studies and found to be acceptable and the percentage recoveries were found in the range of 98.81-100.720 and 99.290-100.700%. The proposed method was successfully validated and applied for the quantitative estimation of these drugs in both bulk and tablet dosage forms. © 2020 Author(s).
URI: http://hdl.handle.net/10995/103319
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85096433367
PURE ID: 20138157
a207b225-ece8-4b11-92f8-a930e5bd908a
ISSN: 0094243X
ISBN: 9780735440104
DOI: 10.1063/5.0018206
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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