Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на этот ресурс:
http://elar.urfu.ru/handle/10995/103110
Название: | Antiviral effect of novel purine conjugate LAS-131 against Herpes simplex virus type 1 (Herpesviridae: Alphaherpesvirinae: Simplexvirus: Human alphaherpesvirus 1) in vitro Антивирусный эффект нового конъюгата пурина LAS-131 в отношении вируса простого герпеса 1 типа (Herpesviridae: Alphaherpesvirinae: Simplexvirus: Human alphaherpesvirus 1) in vitro |
Авторы: | Andronova, V. L. Galegov, G. A. Musiyak, V. V. Vozdvizhenskaya, O. A. Levit, G. L. Krasnov, V. P. |
Дата публикации: | 2021 |
Издатель: | FBSI Central Research Institute of Epidemiology of Rospotrebnadzor |
Библиографическое описание: | Antiviral effect of novel purine conjugate LAS-131 against Herpes simplex virus type 1 (Herpesviridae: Alphaherpesvirinae: Simplexvirus: Human alphaherpesvirus 1) in vitro / V. L. Andronova, G. A. Galegov, V. V. Musiyak, et al. — DOI 10.36233/0507-4088-2020-65-6-8 // Voprosy Virusologii. — 2021. — Vol. 65. — Iss. 6. — P. 373-380. |
Аннотация: | Introduction. Herpes simplex viruses type 1 (HSV-1) are extremely widespread throughout the world and, similar to other herpesviruses, establish lifelong persistent infection in the host. Reactivating sporadically, HSV-1 elicits recurrences in both immunocompetent and immunocompromised individuals and can cause serious diseases (blindness, encephalitis, generalized infections). The currently available antiherpetic drugs that aimed mainly at suppressing replication of viral DNA are not always effective enough, for example, due to the development of drug resistance. As we showed earlier the newly discovered compound LAS-131 exhibits the strong and highly selective inhibitory activity against HSV-1, including strain resistant to acyclovir (selective index, SI = 63). The presence of LAS-131 at a concentration of 20 μg/ml leads to a decrease in the titer of HSV-1 (strain L2) by 4 lg in a one round of HSV-1 replication. Material and methods. To establish the step(s) of the virus life cycle that is sensitive to the action of LAS-131, we have applied a widely used approach, that made it possible to determine how long the addition of a compound can be postponed before it loses its antiviral activity (time-of-addition assay), and to compare this indicator with the crucial time of application of inhibitors with a well-known mechanism of action (in cell culture). Results. It has been shown for the first time that LAS-131 retains a pronounced antiviral effect when introduced into the experimental system no later than 9 hours post-infection (p.i.). However, LAS-131 does not affect the release of HSV-1 from the cell. Discussion. Together with published data on the termination of the synthesis of viral DNA 9–12 h after the adsorption in a cell culture infected with HSV with a high multiplicity (≥1 PFU/cell), our results suggest that LAS-131 interferes the life cycle of HSV-1 during synthesis of viral DNA. Further studies of the mechanism of action are necessary to establish definitely the biological target for this compound,. © 2020 Izdatel'stvo Meditsina. All rights reserved. |
Ключевые слова: | HERPES SIMPLEX VIRUS IN VITRO ANTIVIRAL ACTIVITY MECHANISM OF ACTION TIME-OF-ADDITION ASSAY ACICLOVIR LAS 131 PURINE UNCLASSIFIED DRUG VIRUS DNA ANTIVIRAL ACTIVITY ARTICLE CELL CULTURE HUMAN ALPHAHERPESVIRUS 1 IN VITRO STUDY LIFE CYCLE NONHUMAN VIRUS LOAD VIRUS REPLICATION |
URI: | http://elar.urfu.ru/handle/10995/103110 |
Условия доступа: | info:eu-repo/semantics/openAccess |
Идентификатор РИНЦ: | 44488671 |
Идентификатор SCOPUS: | 85100305096 |
Идентификатор PURE: | 20522584 a11298b0-b7d4-45d8-98a5-27536e0956b0 |
ISSN: | 5074088 |
DOI: | 10.36233/0507-4088-2020-65-6-8 |
Располагается в коллекциях: | Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC |
Файлы этого ресурса:
Файл | Описание | Размер | Формат | |
---|---|---|---|---|
2-s2.0-85100305096.pdf | 611,16 kB | Adobe PDF | Просмотреть/Открыть |
Все ресурсы в архиве электронных ресурсов защищены авторским правом, все права сохранены.