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http://elar.urfu.ru/handle/10995/102947
Title: | Electrochemical dna sensor based on poly(Azure a) obtained from the buffer saturated with chloroform |
Authors: | Porfireva, A. Plastinina, K. Evtugyn, V. Kuzin, Y. Evtugyn, G. |
Issue Date: | 2021 |
Publisher: | MDPI AG |
Citation: | Electrochemical dna sensor based on poly(Azure a) obtained from the buffer saturated with chloroform / A. Porfireva, K. Plastinina, V. Evtugyn, et al. — DOI 10.3390/s21092949 // Sensors. — 2021. — Vol. 21. — Iss. 9. — 2949. |
Abstract: | Electropolymerized redox polymers offer broad opportunities in detection of biospecific interactions of DNA. In this work, Azure A was electrochemically polymerized by multiple cycling of the potential in phosphate buffer saturated with chloroform and applied for discrimination of the DNA damage. The influence of organic solvent on electrochemical properties of the coating was quantified and conditions for implementation of DNA in the growing polymer film were assessed using cyclic voltammetry, quartz crystal microbalance, and electrochemical impedance spectros-copy. As shown, both chloroform and DNA affected the morphology of the polymer surface and electropolymerization efficiency. The electrochemical DNA sensor developed made it possible to distinguish native and thermally and chemically damaged DNA by changes in the charge transfer resistance and capacitance. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
Keywords: | AZURE A DNA DAMAGE DETECTION ELECTROCHEMICAL DNA SENSOR ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY ELECTROPOLYMERIZATION CHARGE TRANSFER CHLORINE COMPOUNDS CYCLIC VOLTAMMETRY ELECTROPOLYMERIZATION MORPHOLOGY POLYMER FILMS SEMICONDUCTING FILMS BIOSPECIFIC INTERACTION CHARGE TRANSFER RESISTANCE DNA DAMAGES ELECTROCHEMICAL DNA SENSOR ELECTROCHEMICAL IMPEDANCE PHOSPHATE BUFFERS POLYMER SURFACES REDOX POLYMERS DNA AZURE A CHLOROFORM DNA POLYMER GIEMSA STAIN AZURE STAINS CHLOROFORM DNA POLYMERS |
URI: | http://elar.urfu.ru/handle/10995/102947 |
Access: | info:eu-repo/semantics/openAccess |
SCOPUS ID: | 85104518578 |
WOS ID: | 000650770900001 |
PURE ID: | 21870861 2d30c32b-06bf-4e36-8d14-49d05e811012 |
ISSN: | 14248220 |
DOI: | 10.3390/s21092949 |
metadata.dc.description.sponsorship: | This research was funded by Russian Science Foundation, grant number 17‐73‐20024. |
RSCF project card: | 17-73-20024 |
Appears in Collections: | Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC |
Files in This Item:
File | Description | Size | Format | |
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2-s2.0-85104518578.pdf | 6,7 MB | Adobe PDF | View/Open |
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