Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/102947
Title: Electrochemical dna sensor based on poly(Azure a) obtained from the buffer saturated with chloroform
Authors: Porfireva, A.
Plastinina, K.
Evtugyn, V.
Kuzin, Y.
Evtugyn, G.
Issue Date: 2021
Publisher: MDPI AG
Citation: Electrochemical dna sensor based on poly(Azure a) obtained from the buffer saturated with chloroform / A. Porfireva, K. Plastinina, V. Evtugyn, et al. — DOI 10.3390/s21092949 // Sensors. — 2021. — Vol. 21. — Iss. 9. — 2949.
Abstract: Electropolymerized redox polymers offer broad opportunities in detection of biospecific interactions of DNA. In this work, Azure A was electrochemically polymerized by multiple cycling of the potential in phosphate buffer saturated with chloroform and applied for discrimination of the DNA damage. The influence of organic solvent on electrochemical properties of the coating was quantified and conditions for implementation of DNA in the growing polymer film were assessed using cyclic voltammetry, quartz crystal microbalance, and electrochemical impedance spectros-copy. As shown, both chloroform and DNA affected the morphology of the polymer surface and electropolymerization efficiency. The electrochemical DNA sensor developed made it possible to distinguish native and thermally and chemically damaged DNA by changes in the charge transfer resistance and capacitance. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords: AZURE A
DNA DAMAGE DETECTION
ELECTROCHEMICAL DNA SENSOR
ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
ELECTROPOLYMERIZATION
CHARGE TRANSFER
CHLORINE COMPOUNDS
CYCLIC VOLTAMMETRY
ELECTROPOLYMERIZATION
MORPHOLOGY
POLYMER FILMS
SEMICONDUCTING FILMS
BIOSPECIFIC INTERACTION
CHARGE TRANSFER RESISTANCE
DNA DAMAGES
ELECTROCHEMICAL DNA SENSOR
ELECTROCHEMICAL IMPEDANCE
PHOSPHATE BUFFERS
POLYMER SURFACES
REDOX POLYMERS
DNA
AZURE A
CHLOROFORM
DNA
POLYMER
GIEMSA STAIN
AZURE STAINS
CHLOROFORM
DNA
POLYMERS
URI: http://hdl.handle.net/10995/102947
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85104518578
PURE ID: 21870861
2d30c32b-06bf-4e36-8d14-49d05e811012
ISSN: 14248220
DOI: 10.3390/s21092949
metadata.dc.description.sponsorship: This research was funded by Russian Science Foundation, grant number 17‐73‐20024.
RSCF project card: 17-73-20024
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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