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http://elar.urfu.ru/handle/10995/90259
Название: | Impedimetric determination of kanamycin in milk with aptasensor based on carbon black‐oligolactide composite |
Авторы: | Kulikova, T. Gorbatchuk, V. Stoikov, I. Rogov, A. Evtugyn, G. Hianik, T. |
Дата публикации: | 2020 |
Издатель: | MDPI AG |
Библиографическое описание: | Impedimetric determination of kanamycin in milk with aptasensor based on carbon black‐oligolactide composite / T. Kulikova, V. Gorbatchuk, I. Stoikov, A. Rogov, et al. . — DOI 10.3390/s20174738 // Sensors (Switzerland). — 2020. — Vol. 17. — Iss. 20. — P. 1-17. |
Аннотация: | The determination of antibiotics in food is important due to their negative effect on human health related to antimicrobial resistance problem, renal toxicity, and allergic effects. We propose an impedimetric aptasensor for the determination of kanamycin A (KANA), which was assembled on the glassy carbon electrode by the deposition of carbon black in a chitosan matrix followed by carbodiimide binding of aminated aptamer mixed with oligolactide derivative of thiacalix[4]arene in a cone configuration. The assembling was monitored by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. In the presence of the KANA, the charge transfer resistance of the inner interface surprisingly decreased with the analyte concentration within 0.7 and 50 nM (limit of detection 0.3 nM). This was attributed to the partial shielding of the negative charge of the aptamer and of its support, a highly porous 3D structure of the surface layer caused by a macrocyclic core of the carrier. The use of electrostatic assembling in the presence of cationic polyelectrolyte decreased tenfold the detectable concentration of KANA. The aptasensor was successfully tested in the determination of KANA in spiked milk and yogurt with recoveries within 95% and 115%. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
Ключевые слова: | ELECTROCHEMICAL BIOSENSOR ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY KANAMYCIN MILK CONTAMINATION THIACALIX[4]ARENE CHARGE TRANSFER CYCLIC VOLTAMMETRY DAIRIES ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY GLASS MEMBRANE ELECTRODES POLYELECTROLYTES SCANNING ELECTRON MICROSCOPY ANALYTE CONCENTRATION ANTIMICROBIAL RESISTANCES CATIONIC POLYELECTROLYTE CHARGE TRANSFER RESISTANCE CHITOSAN MATRICES GLASSY CARBON ELECTRODES LIMIT OF DETECTION THIACALIX[4]ARENE CARBON BLACK |
URI: | http://elar.urfu.ru/handle/10995/90259 |
Условия доступа: | info:eu-repo/semantics/openAccess cc-by |
Идентификатор SCOPUS: | 85089704310 |
Идентификатор WOS: | 000569584900001 |
Идентификатор PURE: | 13659438 |
ISSN: | 1424-8220 |
DOI: | 10.3390/s20174738 |
Сведения о поддержке: | Russian Science Foundation, RSF: 16‐13‐00005 1/0419/20 Funding: I.S. acknowledges financial support from the Russian Science Foundation (grant no. 16‐13‐00005) in the synthesis and application in the biosensor platform of the oligolactides bearing thiacalix[4]arene fragments. T.H. acknowledges funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Sklodowska‐Curie grant agreement no. 690898 and from the Science Grant Agency VEGA, project No.1/0419/20. |
Карточка проекта РНФ: | 16-13-00005 |
Располагается в коллекциях: | Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC |
Файлы этого ресурса:
Файл | Описание | Размер | Формат | |
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10.3390-s20174738.pdf | 3,11 MB | Adobe PDF | Просмотреть/Открыть |
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