A chronoamperometric method for determining total antioxidant activity

Abstract

A novel version of a chronoamperometric method of antioxidant determination is described. It is based on the use of the oxidation current of ferrocyanide formed in the reaction of antioxidants to be determined with potassium ferricyanide as an analytical signal. So the total concentration of all antioxidants present in the analyzed solution, i.e., total antioxidant capacity, is measured. The results of blood plasma analysis by the newly presented and certified potentiometry method are in good agreement with each other, R 2 = 0.9980. The detection limit makes 2 × 10 -6 M for uric and ascorbic acids and 5 × 10 -6 M for cysteine and glutathione. © Pleiades Publishing, Ltd., 2012.