Хромато-масс-спектрометрическое определение тиодиуксусной кислоты в моче

Abstract

Оптимизирована пробоподготовка мочи для ГХ-МС определения тиодиуксусной кислоты в диапазоне от 0.1 до 10 мкг/см

We used the gas chromatograph Agilent 7890A with a flame ionization detector and gas chromatography-mass spectrometer Agilent 5975C in the present work. Dimethyl ester of thiodiacetic acid was identified by the absolute retention time (10.36 min), and the ratio of the peak intensities of the ions 146 (primary) and 178 (confirmation). The calibration curve is characterized by a linear dependence of the chromatographic peak area for the ion at m / z 146 of the mass concentration of the analyte in the range of 0.1 – 10 μg/sm3. Optimal chromatographic separation of the mixture components and a sufficiently high detection sensitivity can be achieved with a temperature gradient and splitless. To combine the derivatization and extraction in a single container with a minimum flow rate of organic solvents used chromatographic vial capacity of 1.5 cm