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Название: Electrochemical Sensor for the Detection of Serine β-Lactamase Catalytic Activity
Авторы: Shumyantseva, V. V.
Bulko, T. V.
Presnova, G. V.
Grigorenko, V. G.
Rubtsova, M. Yu.
Дата публикации: 2024
Издатель: Уральский федеральный университет
Ural Federal University
Библиографическое описание: Electrochemical Sensor for the Detection of Serine β-Lactamase Catalytic Activity / V. V. Shumyantseva, T. V. Bulko, G. V. Presnova, V. G. Grigorenko, M. Yu. Rubtsova // Chimica Techno Acta. — 2024. — Vol. 11, No. 4. — № 202411407.
Аннотация: A new approach is proposed based on the use of electrodes modified with carbon nanomaterials to determine enzymatic activity and screening for inhibitors of serine β-lactamases such as extended spectrum β-lactamases (ESBLs). These enzymes are responsible for the development of antibiotic resistance of pathogenic bacteria to β-lactam antibiotics. Electrochemical oxidation of cephalosporin antibiotic cefotaxime was effectively registered at a potential E from +596 to +625 mV (relative to Ag/AgCl). This property makes it possible to determine the change in cefotaxime concentration in solution upon interaction with serine β-lactamases. By analyzing the electrochemical characteristics of the cefotaxime oxidation reaction, the kinetic parameters of its hydrolysis catalyzed by the serine β-lactamase CTXM-116 were determined. The Michaelis constant was KM = 50 μM and the maximum rate of the catalytic reaction was 1.67∙10–6 M/min. A comparative analysis of the electrochemical parameters of the enzyme/substrate cefotaxime and enzyme/substrate cefotaxime/inhibitor sulbactam (SBT) systems was carried out. Inhibition of β-lactamase by sulbactam was characterized by an IC50 value of 2.5 μM. The proposed approach can be used for screening new substrates and inhibitors of β-lactamases.
Ключевые слова: ELECTROANALYSIS
NANOSTRUCTURED SCREEN-PRINTED
ELECTRODES Β-LACTAMASE
CEFOTAXIME
SULBACTAM
BIOELECTROCHEMISTRY
URI: http://elar.urfu.ru/handle/10995/140712
Идентификатор РИНЦ: https://elibrary.ru/item.asp?id=79367704
ISSN: 2411-1414
DOI: 10.15826/chimtech.2024.11.4.07
Сведения о поддержке: The work was carried out within the framework of the Program of fundamental scientific research in the Russian Federation for the long-term period 2021-2030 (No. 122030100168-2).
The authors express their deep gratitude to Dr. N.K. Fursova (State Scientific Center of Applied Microbiology and Biotechnology, Obolensk) for providing an expression vector used for preparation of recombinant β-lactamase CTX-M-116.
Источники: Chimica Techno Acta. 2024. Vol. 11. № 4
Располагается в коллекциях:Chimica Techno Acta

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