COMPARATIVE ANALYSIS AND OPTIMIZATION OF GREEN EXTRACTION TECHNOLOGIES FOR ISOFLAVONES FROM PLANT RAW MATERIALS

Abstract

Isoflavones, a significant class of plant polyphenols, are recognized for their health benefits, including cholesterol reduction, cancer prevention, and mitigation of osteoporosis and menopause symptoms. This study focuses on optimizing the supercritical fluid extraction (SFE) of isoflavones from kudzu (Pueraria montana) and red clover (Trifolium pratense) flowers, which are rich in these compounds. The extraction process was optimized by varying pressure, time, and co-solvent types. Comparisons were made with ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES) and conventional extraction methods to evaluate yield, antioxidant activities, total phenolic content, and total flavonoid content in the extracts.