New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley)

Darkazanli, M.; Kiseleva, I.

doi:10.1063/5.0069149

Please use this identifier to cite or link to this item: http://elar.urfu.ru/handle/10995/132425

Title:	New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley)
Authors:	Darkazanli, M. Kiseleva, I.
Issue Date:	2021
Publisher:	American Institute of Physics Inc.
Citation:	Darkazanli, M & Kiseleva, I 2021, New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley). в GV Zyryanov, S Santra & OS Taniya (ред.), Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020: Proceedings of the IV International Conference., 040010, AIP Conference Proceedings, Том. 2388, American Institute of Physics Inc., 4th International Conference on Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020, Yekaterinburg, Российская Федерация, 16/11/2020. https://doi.org/10.1063/5.0069149 Darkazanli, M., & Kiseleva, I. (2021). New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley). в G. V. Zyryanov, S. Santra, & O. S. Taniya (Ред.), Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020: Proceedings of the IV International Conference [040010] (AIP Conference Proceedings; Том 2388). American Institute of Physics Inc.. https://doi.org/10.1063/5.0069149
Abstract:	The aim of this study was to the isolate the endophytic bacteria, optimize its isolation procedure. Ethanol, sodium hypochlorite, and mercuric chloride at various concentrations and duration were employed to optimize the surface sterilization for the isolation of endophytes from Phaseolus vulgaris, Pisum sativum, and Hordeum vulgare. A total of 21 endophytic bacteria have been isolated from three plants. Combination of 2% sodium hypochlorite, 70% ethanol, and 0.1% mercuric chloride was found effective for the surface sterilization of Phaseolus vulgaris and Pisum sativum. In case of Hordeum vulgare, 70% ethanol and 2% sodium hypochlorite was found suitable for the surface sterilization. Ethanol, sodium hypochlorite, and mercuric chloride were found effective decontaminating agents in optimum condition. © 2021 Author(s).
Keywords:	CORE LOSS FIELD-ORIENTED CONTROL INDUCTION MACHINE OPTIMIZED CONTROL SUCKER ROD PUMP VARIABLE SPEED DRIVE
URI:	http://elar.urfu.ru/handle/10995/132425
Access:	info:eu-repo/semantics/openAccess cc-by-nc
Conference name:	16 November 2020 through 20 November 2020
Conference date:	4th International Conference on Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020
RSCI ID:	49872425
SCOPUS ID:	85120405728
WOS ID:	001123654800013
PURE ID:	860eba00-8eef-4ecc-aa57-44f11d843db9 29069373
ISSN:	0094-243X
ISBN:	978-073544142-2
DOI:	10.1063/5.0069149
Sponsorship:	Department of Science and Technology, Ministry of Science and Technology, India, डीएसटी, (ʋ 19-516-45006) Russian Foundation for Basic Research, РФФИ Government Council on Grants, Russian Federation Funding text 1: The work was funded by RFBR and DST according to the research project ʋ 19-516-45006. The authors also Funding text 2: acknowledge financial support by the Government of the Russian Federation, Act 211, contract ʋ 02.A03.21.0006.
Appears in Collections:	Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC

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