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Название: Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent
Авторы: Porfireva, A.
Begisheva, E.
Evtugyn, V.
Evtugyn, G.
Дата публикации: 2023
Издатель: Multidisciplinary Digital Publishing Institute (MDPI)
Библиографическое описание: Porfireva, A, Begisheva, E, Evtugyn, V & Evtugyn, G 2023, 'Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent', Biosensors, Том. 13, № 10, 931. https://doi.org/10.3390/bios13100931
Porfireva, A., Begisheva, E., Evtugyn, V., & Evtugyn, G. (2023). Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent. Biosensors, 13(10), [931]. https://doi.org/10.3390/bios13100931
Аннотация: A novel electrochemical DNA sensor was developed for the detection of the anthracycline drug, valrubicin, on the base of poly(Azure C) electropolymerized from the deep eutectic solvent reline and covered with adsorbed DNA from calf thymus. Biosensor assembling was performed by multiple scanning of the potential in one drop (100 µL) of the dye dissolved in reline and placed on the surface of a screen-printed carbon electrode. Stabilization of the coating was achieved by its polarization in the phosphate buffer. The electrochemical characteristics of the electron transfer were determined and compared with a similar coating obtained from phosphate buffer. The use of deep eutectic solvent made it possible to increase the monomer concentration and avoid using organic solvents on the stage of electrode modification. After the contact of the DNA sensor with valrubicin, two signals related to the intrinsic redox activity of the coating and the drug redox conversion were found on voltammogram. Their synchronous changes with the analyte concentration increased the reliability of the detection. In the square-wave mode, the DNA sensor made it possible to determine from 3 µM to 1 mM (limit of detection, 1 µM) in optimal conditions. The DNA sensor was successfully tested in the voltammetric determination of valrubicin in spiked artificial urine, Ringer-Locke solution mimicking plasma electrolytes and biological samples (urine and saliva) with a recovery of 90–110%. After further testing on clinical samples, it can find application in the pharmacokinetics studies and screening of new drugs’ interaction with DNA. © 2023 by the authors.
Ключевые слова: AZURE C
DEEP EUTECTIC SOLVENT
DNA SENSOR
ELECTROCHEMICAL SENSOR
ELECTROPOLYMERIZATION
VALRUBICIN DETERMINATION
BIOSENSORS
CHEMICAL DETECTION
DNA
ELECTROCHEMICAL BIOSENSORS
ELECTROCHEMICAL ELECTRODES
ELECTROPOLYMERIZATION
EUTECTICS
REDOX REACTIONS
SOLVENTS
ANTHRACYCLINE DRUGS
AZURE C
C FILMS
DEEP EUTECTIC SOLVENTS
DNA SENSORS
ELECTROCHEMICAL DNA SENSOR
ELECTROPOLYMERISATION
PHOSPHATE BUFFERS
VALRUBICIN
VALRUBICIN DETERMINATION
COATINGS
ANTHRACYCLINE
DEEP EUTECTIC SOLVENT
REACTIVE OXYGEN METABOLITE
VALRUBICIN
AZURE C
DNA
PHOSPHATE
SOLVENT
VALRUBICIN
ARTICLE
CONTROLLED STUDY
CYCLIC VOLTAMMETRY
DRUG DETERMINATION
ELECTROCHEMICAL ANALYSIS
ELECTROLYTE BLOOD LEVEL
ELECTRON TRANSPORT
HUMAN
LIMIT OF DETECTION
PH
PHARMACOKINETICS
POLYMERIZATION
SCANNING ELECTRON MICROSCOPY
STOICHIOMETRY
THYMUS
VOLTAMMETRY
REPRODUCIBILITY
DEEP EUTECTIC SOLVENTS
DNA
ELECTROCHEMICAL TECHNIQUES
ELECTRODES
PHOSPHATES
REPRODUCIBILITY OF RESULTS
SOLVENTS
URI: http://elar.urfu.ru/handle/10995/130894
Условия доступа: info:eu-repo/semantics/openAccess
cc-by
Текст лицензии: https://creativecommons.org/licenses/by/4.0/
Идентификатор SCOPUS: 85175077994
Идентификатор WOS: 001094181200001
Идентификатор PURE: 47596922
ISSN: 2079-6374
DOI: 10.3390/bios13100931
Сведения о поддержке: Russian Science Foundation, RSF: 23-13-00163
This research was funded by the Russian Science Foundation (grant No. 23-13-00163), https://www.rscf.ru/en/project/23-13-00163 (assessed on 17 August 2023).
Карточка проекта РНФ: 23-13-00163
Располагается в коллекциях:Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC

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