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http://elar.urfu.ru/handle/10995/118086
Название: | A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets |
Авторы: | Tikhonova, M. A. Maslov, N. A. Bashirzade, A. A. Nehoroshev, E. V. Babchenko, V. Y. Chizhova, N. D. Tsibulskaya, E. O. Akopyan, A. A. Markova, E. V. Yang, Y. -L. Lu, K. -T. Kalueff, A. V. Aftanas, L. I. Amstislavskaya, T. G. |
Дата публикации: | 2022 |
Издатель: | MDPI |
Библиографическое описание: | A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets / M. A. Tikhonova, N. A. Maslov, A. A. Bashirzade et al. // Pharmaceutics. — 2022. — Vol. 14. — Iss. 8. — 1751. |
Аннотация: | Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening. © 2022 by the authors. |
Ключевые слова: | ANIMAL MODEL BEHAVIOR LASER NEURODEGENERATION NEUROINFLAMMATION NEUROREPAIR TRAUMATIC BRAIN INJURY ZEBRAFISH BRAIN DERIVED NEUROTROPHIC FACTOR CELL PROTEIN HYDROCORTISONE HYPOXIA INDUCIBLE FACTOR 1ALPHA IONIZED CALCIUM BINDING ADAPTER MOLECULE 1 PROTEIN NEURON SPECIFIC NUCLEAR PROTEIN UNCLASSIFIED DRUG ADULT ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE ANXIETY DISORDER ARTICLE BEHAVIOR ASSESSMENT CELL ACTIVATION CELL DAMAGE CONSTRUCT VALIDITY CONTROLLED STUDY CONVALESCENCE FACE VALIDITY FEMALE HYPOLOCOMOTION LOCOMOTION MALE MICROGLIA MOLECULARLY TARGETED THERAPY MORPHOLOGY NERVOUS SYSTEM INFLAMMATION NEUROMORPHOLOGY NONHUMAN NOVEL TANK TEST PROTEIN EXPRESSION TELENCEPHALON TRAUMATIC BRAIN INJURY VELOCITY ZEBRA FISH |
URI: | http://elar.urfu.ru/handle/10995/118086 |
Условия доступа: | info:eu-repo/semantics/openAccess |
Идентификатор SCOPUS: | 85137388324 |
Идентификатор WOS: | 000845798700001 |
Идентификатор PURE: | 30897484 |
ISSN: | 19994923 |
DOI: | 10.3390/pharmaceutics14081751 |
Сведения о поддержке: | Saint Petersburg State University, SPbU: 73026081; Russian Science Foundation, RSF: 20-65-46006 We thank Alisa S. Belova for technical support in experimental manipulations and cortisol assay. We also thank Anatoly A. Maslov for the idea of using laser radiation to introduce brain damage. A.V.K. lab is supported by St. Peterburg State University funds (Pure ID 73026081). This study was funded by Russian Science Foundation (grant No. 20-65-46006). |
Карточка проекта РНФ: | 20-65-46006 |
Располагается в коллекциях: | Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC |
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Файл | Описание | Размер | Формат | |
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2-s2.0-85137388324.pdf | 27,13 MB | Adobe PDF | Просмотреть/Открыть |
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