Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/118056
Title: Biological Impact of γ-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications
Authors: Fadeyev, F. A.
Blyakhman, F. A.
Safronov, A. P.
Melnikov, G. Y.
Nikanorova, A. D.
Novoselova, I. P.
Kurlyandskaya, G. V.
Issue Date: 2022
Publisher: MDPI
Citation: Biological Impact of γ-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications / F. A. Fadeyev, F. A. Blyakhman, A. P. Safronov et al. // Biosensors. — 2022. — Vol. 12. — Iss. 8. — 627.
Abstract: The biological activity of γ-Fe2O3 magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro on the primary cultures of human dermal fibroblasts. The effects of the MNPs contained in culture medium or MNPs already uptaken by cells were evaluated for the cases of the fibroblast’s proliferation and secretion of cytokines and collagen. For the tests related to the contribution of the constant magnetic field to the biological activity of MNPs, a magnetic system for the creation of the external magnetic field (having no commercial analogues) was designed, calibrated, and used. It was adapted to the size of standard 24-well cell culture plates. At low concentrations of MNPs, uptake by fibroblasts had stimulated their proliferation. Extracellular MNPs stimulated the release of pro-inflammatory cytokines (Interleukin-6 (IL-6) and Interleukin-8 (IL-8) or chemokine (C-X-C motif) ligand 8 (CXCL8)) in a concentration-dependent manner. However, the presence of MNPs did not increase the collagen secretion. The exposure to the uniform constant magnetic field (H ≈ 630 or 320 Oe), oriented in the plane of the well, did not cause considerable changes in fibroblasts proliferation and secretion, regardless of presence of MNPs. Statistically significant differences were detected only in the levels of IL-8/CXCL8 release. © 2022 by the authors.
Keywords: BIOMEDICAL APPLICATIONS
CELL PROLIFERATION
CONSTANT MAGNETIC FIELD
CYTOKINE SECRETION
HUMAN SKIN FIBROBLASTS
MAGNETIC NANOPARTICLES
COLLAGEN
CYTOKINE
INTERLEUKIN 6
INTERLEUKIN 8
IRON OXIDE NANOPARTICLE
MAGNETIC NANOPARTICLE
COLLAGEN
INTERLEUKIN 8
MAGNETITE NANOPARTICLE
ARTICLE
BIOLOGICAL ACTIVITY
BIOMEDICINE
CALIBRATION
CELL PROLIFERATION
CELL STIMULATION
CONCENTRATION (PARAMETER)
CONTROLLED STUDY
CYTOKINE RELEASE
EVAPORATION
HUMAN
HUMAN CELL
HUMAN CELL CULTURE
IN VITRO STUDY
MAGNETIC FIELD
MAGNETISM
PROTEIN SECRETION
SKIN FIBROBLAST
SYNTHESIS
GENETIC PROCEDURES
LASER
BIOSENSING TECHNIQUES
COLLAGEN
HUMANS
INTERLEUKIN-8
LASERS
MAGNETIC PHENOMENA
MAGNETITE NANOPARTICLES
URI: http://hdl.handle.net/10995/118056
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85136510365
ISSN: 20796374
DOI: 10.3390/bios12080627
metadata.dc.description.sponsorship: IT1245-19; Ministry of Science and Higher Education of the Russian Federation: FEUZ-2020-0051; Ministry of Health of the Russian Federation: 121032300335-1
The study was supported by the program of the Ministry of Health of the Russian Federation (project 121032300335-1). This work was financially supported, in part, by the Ministry of Science and Higher Education of the RF (grant FEUZ-2020-0051) (G.Yu. Melnikov) and University of the Basque Country Research Groups Funding (grant IT1245-19) (G.V. Kurlyandskaya).
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