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http://elar.urfu.ru/handle/10995/118056
Название: | Biological Impact of γ-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications |
Авторы: | Fadeyev, F. A. Blyakhman, F. A. Safronov, A. P. Melnikov, G. Y. Nikanorova, A. D. Novoselova, I. P. Kurlyandskaya, G. V. |
Дата публикации: | 2022 |
Издатель: | MDPI |
Библиографическое описание: | Biological Impact of γ-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications / F. A. Fadeyev, F. A. Blyakhman, A. P. Safronov et al. // Biosensors. — 2022. — Vol. 12. — Iss. 8. — 627. |
Аннотация: | The biological activity of γ-Fe2O3 magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro on the primary cultures of human dermal fibroblasts. The effects of the MNPs contained in culture medium or MNPs already uptaken by cells were evaluated for the cases of the fibroblast’s proliferation and secretion of cytokines and collagen. For the tests related to the contribution of the constant magnetic field to the biological activity of MNPs, a magnetic system for the creation of the external magnetic field (having no commercial analogues) was designed, calibrated, and used. It was adapted to the size of standard 24-well cell culture plates. At low concentrations of MNPs, uptake by fibroblasts had stimulated their proliferation. Extracellular MNPs stimulated the release of pro-inflammatory cytokines (Interleukin-6 (IL-6) and Interleukin-8 (IL-8) or chemokine (C-X-C motif) ligand 8 (CXCL8)) in a concentration-dependent manner. However, the presence of MNPs did not increase the collagen secretion. The exposure to the uniform constant magnetic field (H ≈ 630 or 320 Oe), oriented in the plane of the well, did not cause considerable changes in fibroblasts proliferation and secretion, regardless of presence of MNPs. Statistically significant differences were detected only in the levels of IL-8/CXCL8 release. © 2022 by the authors. |
Ключевые слова: | BIOMEDICAL APPLICATIONS CELL PROLIFERATION CONSTANT MAGNETIC FIELD CYTOKINE SECRETION HUMAN SKIN FIBROBLASTS MAGNETIC NANOPARTICLES COLLAGEN CYTOKINE INTERLEUKIN 6 INTERLEUKIN 8 IRON OXIDE NANOPARTICLE MAGNETIC NANOPARTICLE COLLAGEN INTERLEUKIN 8 MAGNETITE NANOPARTICLE ARTICLE BIOLOGICAL ACTIVITY BIOMEDICINE CALIBRATION CELL PROLIFERATION CELL STIMULATION CONCENTRATION (PARAMETER) CONTROLLED STUDY CYTOKINE RELEASE EVAPORATION HUMAN HUMAN CELL HUMAN CELL CULTURE IN VITRO STUDY MAGNETIC FIELD MAGNETISM PROTEIN SECRETION SKIN FIBROBLAST SYNTHESIS GENETIC PROCEDURES LASER BIOSENSING TECHNIQUES COLLAGEN HUMANS INTERLEUKIN-8 LASERS MAGNETIC PHENOMENA MAGNETITE NANOPARTICLES |
URI: | http://elar.urfu.ru/handle/10995/118056 |
Условия доступа: | info:eu-repo/semantics/openAccess |
Идентификатор SCOPUS: | 85136510365 |
Идентификатор WOS: | 000847129900001 |
Идентификатор PURE: | 30851250 |
ISSN: | 20796374 |
DOI: | 10.3390/bios12080627 |
Сведения о поддержке: | IT1245-19; Ministry of Science and Higher Education of the Russian Federation: FEUZ-2020-0051; Ministry of Health of the Russian Federation: 121032300335-1 The study was supported by the program of the Ministry of Health of the Russian Federation (project 121032300335-1). This work was financially supported, in part, by the Ministry of Science and Higher Education of the RF (grant FEUZ-2020-0051) (G.Yu. Melnikov) and University of the Basque Country Research Groups Funding (grant IT1245-19) (G.V. Kurlyandskaya). |
Располагается в коллекциях: | Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC |
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2-s2.0-85136510365.pdf | 4,26 MB | Adobe PDF | Просмотреть/Открыть |
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