Synthesis, in Vitro and Docking Studies of 2-substituted 5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidine-4(3H)-one Derivatives as Agents for the treatment of Alzheimer's Disease

Abstract

Alzheimer's disease is a chronic neurodegenerative disease, which is characterized mainly by a progressive decrease in intellectual abilities, memory impairment and a change in a person's personality. Unfortunately, there are practically no medicines that act on the pathogenesis of Alzheimer's disease. The development of new highly effective medicines for the treatment of this pathology is one of the crucial areas of pharmaceutical research. The aim of this work is to search among 2-substituted 5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3- d]pyrimidine-4(3H)-one effective compounds with anticholinesterase and antiamyloid activities. As a result, it was found that compounds 4d, 4e and 4f have the highest anticholinesterase ability, containing in their structure the residues of hydroxy-methoxyphenyl fragments. Structures 4c, 4g, 4h, 4j, 4k, 4m, 4n and 4p showed slightly lower activity, the effect of which did not differ statistically from that of Donepezil. Compounds 4c, 4e, 4k and 4m have the greatest ability to inhibit the formation of the amyloid, comparable to GV-971. It should be noted that the molecular docking data are consistent with the results of the determination of the anticholinesterase activity of the studied compounds obtained in vitro. Thus, the prospects for future studies of these compounds concerning the possibility of creating a pharmaceutical active substance for the treatment of neurodegenerative diseases have been revealed.