Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/112253
Title: Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea Deltoidea, Tribulus Terrestris and Panax Japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus
Authors: Povydysh, M. N.
Titova, M. V.
Ivanov, I. M.
Klushin, A. G.
Kochkin, D. V.
Galishev, B. A.
Popova, E. V.
Ivkin, D. Yu.
Luzhanin, V. G.
Krasnova, M. V.
Demakova, N. V.
Nosov, A. M.
Issue Date: 2021
Publisher: MDPI
MDPI AG
Citation: Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea Deltoidea, Tribulus Terrestris and Panax Japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus / M. N. Povydysh, M. V. Titova, I. M. Ivanov et al. // Nutrients. — 2021. — Vol. 13. — Iss. 11. — 3811.
Abstract: In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species Dioscorea deltoidea, Tribulus terrestris and Panax japonicus to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1–2.5 h (D. deltoidea and T. terrestris) or 1 h (P. japonicus) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of D. deltoidea and T. terrestris acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39– 57%), blood-glucose level (by 46–51%), blood content in urine (by 78–80%) and total cholesterol (25– 36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in D. deltoidea; furostanol-type steroidal glycosides and quinic acid derivatives in T. terrestris; and ginsenosides and malonyl-ginsenosides in P. japonicus. These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This.
Keywords: ADRENALINE MODEL
BIOREACTORS
FUROSTANOL-TYPE GLYCOSIDES
HYPERGLYCEMIA
PLANT CELL CULTURE
STREPTOZOTOCIN-INDUCED TYPE 2 DIABETES MELLITUS
CHOLESTEROL
PLANT EXTRACT
ANIMAL
BIOMASS
BIOREACTOR
BLOOD
CARBOHYDRATE METABOLISM
CELL CULTURE TECHNIQUE
DIURESIS
DRUG EFFECT
EXPERIMENTAL DIABETES MELLITUS
GLUCOSE BLOOD LEVEL
HEMATURIA
LIPID METABOLISM
MEDICINAL PLANT
NON INSULIN DEPENDENT DIABETES MELLITUS
PANAX
RAT
TRIBULUS
YAM
ANIMALS
BIOMASS
BIOREACTORS
BLOOD GLUCOSE
CARBOHYDRATE METABOLISM
CELL CULTURE TECHNIQUES
CHOLESTEROL
DIABETES MELLITUS, EXPERIMENTAL
DIABETES MELLITUS, TYPE 2
DIOSCOREA
DIURESIS
HEMATURIA
LIPID METABOLISM
PANAX
PLANT EXTRACTS
PLANTS, MEDICINAL
RATS
TRIBULUS
URI: http://hdl.handle.net/10995/112253
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85117941277
PURE ID: 23974064
ISSN: 2072-6643
metadata.dc.description.sponsorship: Funding: Bioreactor cultivation of plant cell suspensions and their biochemical analysis were financially supported by the Ministry of Science and Higher Education of Russian Federation through Megagrant project no. 075-15-2019-1882 and performed by using the equipment of the large-scale research facilities “Experimental biotechnological facility” and “All-Russian Collection of cell cultures of higher plants” of the IPPRAS (EBF IPPRAS and ARCCC HP IPPRAS). Hypoglycemic activity evaluation of the cell biomass was performed with the financial support of the Russian Science Foundation project no. 19-14-00387. The results of the hypoglycemic activity evaluation were obtained by using the equipment of the Center for Collective Use “Analytical Center of the Federal State Budgetary Educational Institution of Higher Education Saint Petersburg State Chemical Pharmaceutical University (SPCPU) of the Ministry of Health of Russia”, equipped with the financial support of the Ministry of Education and Science of Russia.
RSCF project card: 19-14-00387
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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