Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/112242
Title: Naloxone Prolongs Abdominal Constriction Writhing-Like Behavior in a Zebrafish-Based Pain Model
Authors: Costa, F. V.
Canzian, J.
Stefanello, F. V.
Kalueff, A. V.
Rosemberg, D. B.
Issue Date: 2019
Publisher: Elsevier Ireland Ltd
Elsevier BV
Citation: Naloxone Prolongs Abdominal Constriction Writhing-Like Behavior in a Zebrafish-Based Pain Model / F. V. Costa, J. Canzian, F. V. Stefanello et al. // Neuroscience Letters. — 2019. — Vol. 708. — 134336.
Abstract: The ability to detect noxious stimuli is essential to survival. However, pathological pain is maladaptive and severely debilitating. Endogenous and exogenous opioids modulate pain responses via opioid receptors, reducing pain sensibility. Due to the high genetic and physiological similarities to rodents and humans, the zebrafish is a valuable tool to assess pain responses and the underlying mechanisms involved in nociception. Although morphine attenuates pain-like responses of zebrafish, there are no data showing if the antagonism of opioid receptors prolongs pain duration in the absence of an exogenous opioid. Here, we investigated whether a common opioid antagonist naloxone affects the abdominal constriction writhing-like response, recently characterized as a zebrafish-based pain behavior. Animals were injected intraperitoneally with acetic acid (5.0%), naloxone (1.25 mg/kg; 2.5 mg/kg; 5.0 mg/kg) or acetic acid with naloxone to investigate the changes in their body curvature for 1 h. Acetic acid elicited a robust pain-like response in zebrafish, as assessed by aberrant abdominal body curvature, while no effects were observed following PBS injection. Although naloxone alone did not alter the frequency and duration of this behavior, it dose-dependently prolonged acetic acid-induced abdominal curvature response. Besides reinforcing the use of the abdominal writhing-like phenotype as a behavioral endpoint to measure acute pain responses in zebrafish models, our novel data suggest a putative role of endogenous opioids in modulating the recovery from pain stimulation in zebrafish. © 2019 Elsevier B.V.
Keywords: ABDOMINAL CONSTRICTION WRITHING-LIKE BEHAVIOR
ACETIC ACID
NALOXONE
NOCICEPTION
ZEBRAFISH
ACETIC ACID
NALOXONE
OPIATE RECEPTOR
NALOXONE
NARCOTIC ANTAGONIST
ABDOMINAL CONSTRICTION WRITHING LIKE BEHAVIOR
ADULT
ANIMAL EXPERIMENT
ANIMAL MODEL
ARTICLE
BEHAVIOR
CONTROLLED STUDY
DRUG EFFECT
FEMALE
MALE
NOCICEPTION
NONHUMAN
PAIN
PHENOTYPE
PRIORITY JOURNAL
ZEBRA FISH
ABDOMEN
ANIMAL
ANIMAL BEHAVIOR
DISEASE MODEL
PATHOPHYSIOLOGY
PSYCHOLOGY
STENOSIS, OCCLUSION AND OBSTRUCTION
VISCERAL PAIN
ABDOMEN
ACETIC ACID
ANIMALS
BEHAVIOR, ANIMAL
CONSTRICTION, PATHOLOGIC
DISEASE MODELS, ANIMAL
NALOXONE
NARCOTIC ANTAGONISTS
PAIN
VISCERAL PAIN
ZEBRAFISH
URI: http://hdl.handle.net/10995/112242
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85067894326
PURE ID: 10015772
ISSN: 0304-3940
metadata.dc.description.sponsorship: We recognize the financial support and fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) . F.V.C. was recipient of CAPES fellowship. J.C. and F.V.S. receive the CNPq fellowship. D.B.R. is a recipient of CNPq research productivity grant ( 305051/2018-0 ) and his work is also supported by the PROEX/CAPES (process number 23038.005848/2018-31) and PRONEM/FAPERGS (process number 16/2551-0000248-7) fellowship grants. A.V.K. is the Chair of the International Zebrafish Neuroscience Research Consortium (ZNRC). His research is supported by the Russian Science Foundation (RSF) grant 19-15-00053. All authors contributed to the preparation of the manuscript and approved its final version. The funders had no influence on the study design, collection, analysis, and interpretation of data, as well as on writing and submission of this manuscript.
RSCF project card: 19-15-00053
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

Files in This Item:
File Description SizeFormat 
2-s2.0-85067894326.pdf1,49 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.