Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/111772
Title: Microbiota of Semen Samples with Normozoospermia: Analysis of Real-time PCR Data
Other Titles: Микробиота эякулята у пациентов с нормозооспермией по результатам исследования методом ПЦР в реальном времени
Authors: Voroshilina, E. S.
Zornikov, D. L.
Ivanov, A. V.
Pochernikov, D. G.
Panacheva, E. A.
Issue Date: 2021
Publisher: Pirogov Russian National Research Medical University
Pirogov Russian National Research Medical University
Citation: Microbiota of Semen Samples with Normozoospermia: Analysis of Real-time PCR Data [Микробиота эякулята у пациентов с нормозооспермией по результатам исследования методом ПЦР в реальном времени] / E. S. Voroshilina, D. L. Zornikov, A. V. Ivanov et al. — DOI 10.1051/ita:2005002 // Bulletin of Russian State Medical University. — 2021. — Vol. — Iss. 5. — P. 54-61.
Abstract: The analysis of semen microbiota is difficult due to the lack of established criteria for interpretation of microbiological tests. The aim of the study was to determine the stable clusters of semen microbiota analyzed by real-time PCR in samples with normozoospermia. Semen samples of 227 men with normal spermiograms were included in the study. The quantity of total bacterial DNA and at least one group of microorganisms was more than 103 GE/ml in 107 (41.7%) samples. Four stable microbiota clusters with the prevalence of a specific microorganism group were distinguished in these samples: obligate anaerobes (OA) cluster (proportion in the centroid - 81.1%); Lactobacillus spp. cluster (proportion in the centroid - 64.3%); gram-positive facultative anaerobes (GPFA) cluster (proportion in the centroid - 92.5%); Enterobacteriaceae/Enterococcoccus (EE) cluster (proportion in the centroid - 80.8%). The clusters were ranked by frequency of occurrence: OA cluster was the most prevalent (43 (40.2%) of 107), second-most frequent were GPFA-cluster (27 (25.2%)) and Lactobacillus-cluster (22 (20.6%)). EE-dominated cluster was found in 15 (14.0%) cases. © 2021 Pirogov Russian National Research Medical University. All rights reserved.
Keywords: CLUSTER ANALYSIS
NORMOZOOSPERMIA
REAL-TIME PCR
SEMEN ANALYSIS
SEMEN MICROBIOTA
BACTERIAL DNA
DEIONIZED WATER
ADULT
ANAEROBE
ARTICLE
BACTERIAL LOAD
CLUSTER ANALYSIS
CONTROLLED STUDY
ENTEROBACTERIACEAE
HUMAN
HUMAN EXPERIMENT
LACTOBACILLUS
MALE
MICROFLORA
MICROORGANISM
NONHUMAN
NORMOZOOSPERMIA
PREVALENCE
REAL TIME POLYMERASE CHAIN REACTION
SEMEN ANALYSIS
SPERM
SPERMIOGRAM
URI: http://hdl.handle.net/10995/111772
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85120050696
PURE ID: 28959742
ISSN: 2500-1094
DOI: 10.1051/ita:2005002
metadata.dc.description.sponsorship: The authors would like to thank VN Khayutin, director of "Garmonia" Medical Center, for allowing them to conduct the study in the clinic's laboratory department.
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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