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Title: Electrochemical Sensing of Interactions between DNA and Charged Macrocycles
Authors: Kulikova, T.
Padnya, P.
Shiabiev, I.
Rogov, A.
Stoikov, I.
Evtugyn, G.
Issue Date: 2021
Publisher: MDPI
Citation: Electrochemical Sensing of Interactions between DNA and Charged Macrocycles / T. Kulikova, P. Padnya, I. Shiabiev et al. // Chemosensors. — 2021. — Vol. 9. — Iss. 12. — 347.
Abstract: In this work, we investigated aggregation of native DNA and thiacalix[4]arene derivative bearing eight terminal amino groups in cone configuration using various redox probes on the glassy carbon electrode. It was shown that sorption transfer of the aggregates on the surface of the electrode covered with carbon black resulted in changes in electrostatic interactions and diffusional permeability of the surface layer. Such changes alter the signals of ferricyanide ion, methylene green and hydroquinone as redox probes to a degree depending on their specific interactions with DNA and own charge. Inclusion of DNA in the surface layer was independently confirmed by scanning electron microscopy, electrochemical impedance spectroscopy and experiments with doxorubicin as a model intercalator. Thermal denaturing of DNA affected the charge separation on the electrode interface and the signals of redox probes. Using hydroquinone, less sensitive to electrostatic interactions, made it possible to determine from 10 pM to 1.0 nM doxorubicin (limit of detection 3 pM) after 10 min incubation. Stabilizers present in the commercial medications did not alter the signal. The DNA sensors developed can find future application in the assessment of the complexes formed by DNA and macrocycles as delivery agents for small chemical species. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85121800610
PURE ID: 29209841
ISSN: 2227-9040
metadata.dc.description.sponsorship: Funding: This research was funded by Russian Science Foundation (grant no. 21-73-20067).
RSCF project card: 21-73-20067
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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