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Title: Electrochemical Sensing of Idarubicin—DNA Interaction Using Electropolymerized Azure B and Methylene Blue Mediation
Authors: Goida, A.
Kuzin, Y.
Evtugyn, V.
Porfireva, A.
Evtugyn, G.
Hianik, T.
Issue Date: 2022
Publisher: MDPI
Citation: Electrochemical Sensing of Idarubicin—DNA Interaction Using Electropolymerized Azure B and Methylene Blue Mediation / A. Goida, Y. Kuzin, V. Evtugyn et al. // Chemosensors. — 2022. — Vol. 10. — Iss. 1. — 33.
Abstract: A highly sensitive electrochemical DNA sensor for detection of the chemotherapeutic drug idarubicin mediated by Methylene blue (MB) has been developed. DNA from fish sperm has been immobilized at the electropolymerized layers of Azure B. The incorporation of MB into the DNA layers substantially increased the sensor sensitivity. The concentration range for idarubicin determination by cyclic voltammetry was from 1 fM to 0.1 nM, with a limit of detection (LOD) of 0.3 fM. Electrochemical impedance spectroscopy (EIS) in the presence of a redox probe ([Fe(CN)6 ]3−/4− ) allowed for the widening of a linear range of idarubicin detection from 1 fM to 100 nM, retaining LOD 0.3 fM. The DNA sensor has been tested in various real and artificial biological fluids with good recovery ranging between 90–110%. The sensor has been successfully used for impedimetric idarubicin detection in medical preparation Zavedos® . The developed DNA biosensor could be useful for the control of the level of idarubicin during cancer therapy as well as for pharmacokinetics studies. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords: AFM
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85123596243
WOS ID: 000758468200001
PURE ID: 29562104
ISSN: 2227-9040
DOI: 10.3390/chemosensors10010033
metadata.dc.description.sponsorship: Funding: A.P. announces the financial support of Russian Science Foundation (grant 17-73-20024). T.H. is grateful to Science Agency VEGA, project No. 1/0419/20, for financial support.
RSCF project card: 17-73-20024
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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