Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/111324
Title: Biomacromolecules in Recent Phosphate-Shelled Brachiopods: Identification and Characterization of Chitin Matrix
Authors: Agbaje, O. B. A.
Brock, G. A.
Zhang, Z.
Duru, K. C.
Liang, Y.
George, S. C.
Holmer, L. E.
Issue Date: 2021
Publisher: Springer
Springer Science and Business Media LLC
Citation: Biomacromolecules in Recent Phosphate-Shelled Brachiopods: Identification and Characterization of Chitin Matrix / O. B. A. Agbaje, G. A. Brock, Z. Zhang et al. — DOI 10.21538/0134-4889-2020-26-4-290-299 // Journal of Materials Science. — 2021. — Vol. 56. — Iss. 36. — P. 19884-19898.
Abstract: Abstract: Phosphate-shelled brachiopods differ in filter-feeding lifestyle, with Lingula anatina an active infaunal burrower, and Discinisca tenuis a shallow marine epibenthic animal. The shells of these animals are built of organophosphatic constituents, the organic fibres/sheets reinforced with calcium phosphate to provide a sophisticated ultrastructural robustness. This investigation examined the nature of the organic fibres in order to improve understanding of how living organisms produce hierarchically structured biomaterials. Unlike powdered samples commonly used in previous studies, organic fibres were isolated for the first time and the shell fractions were purified, in order to study the content and nature of the biopolymer fibres. Biochemical methods including Calcofluor staining revealed a chitin matrix. Ultrastructural analysis, thermal gravimetric analysis, and spectroscopic analyses show that the core polysaccharide framework is composed of layers of β-chitin sheets and/or fibrils that are coated with a fibrous organic matrix. There is more chitin matrix in the L. anatina shells (26.6 wt.%) compared to the D. tenuis shells (12.9 wt.%). Taken together, the data show that the chitin matrix contributes to increased skeletal strength, making L. anatina highly adapted for life as an active burrower. In comparison, D. tenuis contains less chitin and lives as attached epibenthos in a shallow marine environment. Graphical abstract: [Figure not available: see fulltext.] First spectroscopic evidence of β-chitin sheets in recent organophosphatic brachiopods. © 2021, The Author(s).
Keywords: ANIMALS
BIOPOLYMERS
CALCIUM PHOSPHATE
FIBERS
GRAVIMETRIC ANALYSIS
SHELLS (STRUCTURES)
SPECTROSCOPIC ANALYSIS
THERMOGRAVIMETRIC ANALYSIS
BIOCHEMICAL METHODS
BIOMACROMOLECULES
FILTER-FEEDING
LIVING ORGANISMS
POWDERED SAMPLES
SPECTROSCOPIC EVIDENCE
THERMAL GRAVIMETRIC ANALYSIS
ULTRASTRUCTURAL ANALYSIS
CHITIN
URI: http://hdl.handle.net/10995/111324
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85114853099
PURE ID: 29070303
ISSN: 0022-2461
DOI: 10.21538/0134-4889-2020-26-4-290-299
metadata.dc.description.sponsorship: OBAA is grateful to Uppsala University for support through the VR Project number 2018-03390. The research for this paper was supported by the Swedish Research Council (VR Project no. 2018-03390 to LEH, GAB and SCG), by the National Natural Science Foundation of China (41720104002, 41621003 and 41890844 to NWU members), and by a Zhongjian Yang Scholarship to LEH from the Department of Geology, Northwest University, Xi’an. GAB’s research is also funded by a 1000 Talent Shaanxi Province Fellowship at Northwest University, Xi’an.
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