Видовая дифференциация боррелий методом ПЦР в реальном времени

Abstract

In Russia, Lyme disease caused mainly by Borrelia afzelii, B. garinii and possibly B.miyamotoi is of high clinical and scientific impor­tance. In order to estimate the prevalence of these Borrelia species in ticks, animal tissue and clinical material, a new genotyping approach based on Real-Time PCR was developed. No false-positive results were obtained with prim­ers and probes used. The percent of non-typing samples was 7,28 %. To test the new approach, 151 samples from Arkhangel’sk, Perm and Sverdlovsk regions were investigated. The percent of mixed infections reached 38– 44 %. Surpris­ingly high prevalence of B.miyamotoi was observed, especially in Sverdlovsk region (39 %). No tick infected with only B.miyamotoi was detected.