The use of atomic force microscopy for structural and surface morphological analysis of Fanconi anemia patient fibroblasts before and after exposure to γ-radiation

Abstract

The surface morphological changes of Fanconi anemia patient fibroblasts after exposure to γ–radiation were investigated by AFM and foci immunofluorescence staining. The reorganization of the actin cytoskeleton was found, having resulted in reduction of the membrane stiffness and increase of adhesion in nuclear and lamellipodial regions of the cell.