Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/103382
Title: Effects of constant magnetic field to the proliferation rate of human fibroblasts grown onto different substrates: Tissue culture polystyrene, polyacrylamide hydrogel and ferrogels γ-fe2o3 magnetic nanoparticles
Authors: Blyakhman, F. A.
Melnikov, G. Y.
Makarova, E. B.
Fadeyev, F. A.
Sedneva-Lugovets, D. V.
Shabadrov, P. A.
Volchkov, S. O.
Mekhdieva, K. R.
Safronov, A. P.
Armas, S. F.
Kurlyandskaya, G. V.
Issue Date: 2020
Publisher: MDPI AG
Citation: Effects of constant magnetic field to the proliferation rate of human fibroblasts grown onto different substrates: Tissue culture polystyrene, polyacrylamide hydrogel and ferrogels γ-fe2o3 magnetic nanoparticles / F. A. Blyakhman, G. Y. Melnikov, E. B. Makarova, et al. — DOI 10.3390/nano10091697 // Nanomaterials. — 2020. — Vol. 10. — Iss. 9. — P. 1-20. — 1697.
Abstract: The static magnetic field was shown to affect the proliferation, adhesion and differentiation of various types of cells, making it a helpful tool for regenerative medicine, though the mechanism of its impact on cells is not completely understood. In this work, we have designed and tested a magnetic system consisting of an equidistant set of the similar commercial permanent magnets (6 × 4 assay) in order to get insight on the potential of its experimental usage in the biological studies with cells culturing in a magnetic field. Human dermal fibroblasts, which are widely applied in regenerative medicine, were used for the comparative study of their proliferation rate on tissue culture polystyrene (TCPS) and on the polyacrylamide ferrogels with 0.00, 0.63 and 1.19 wt % concentrations of γ-Fe2O3 magnetic nanoparticles obtained by the well-established technique of laser target evaporation. We used either the same batch as in previously performed but different biological experiments or the same fabrication conditions for fabrication of the nanoparticles. This adds special value to the understanding of the mechanisms of nanoparticles contributions to the processes occurring in the living systems in their presence. The magnetic field increased human dermal fibroblast cell proliferation rate on TCPS, but, at the same time, it suppressed the growth of fibroblasts on blank gel and on polyacrylamide ferrogels. However, the proliferation rate of cells on ferrogels positively correlated with the concentration of nanoparticles. Such a dependence was observed both for cell proliferation without the application of the magnetic field and under the exposure to the constant magnetic field. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords: CELL CULTURING
CELL PROLIFERATION
COMSOL MODELLING
HUMAN DERMAL FIBROBLASTS
MAGNETIC FIELD
MAGNETIC NANOPARTICLES
MAGNETIC PROPERTIES
POLYACRYLAMIDE HYDROGELS AND FERROGELS
URI: http://hdl.handle.net/10995/103382
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85093895833
PURE ID: 14149508
636b7701-544b-48e7-8b7d-282c85e4b777
ISSN: 20794991
DOI: 10.3390/nano10091697
metadata.dc.description.sponsorship: The Russian Scientific Foundation (grant 18-19-00090) supported the experimental parts of this study, including the design, performance and analysis of experiments.
RSCF project card: 18-19-00090
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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