Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/103233
Title: Semen microbiota: Cluster analysis of real-time PCR data
МИКРОБИОТА ЭЯКУЛЯТА: КЛАСТЕРНЫЙ АНАЛИЗ РЕЗУЛЬТАТОВ, ПОЛУЧЕННЫХ ПРИ ИССЛЕДОВАНИИ МЕТОДОМ ПЦР-РВ
Authors: Voroshilina, E. S.
Zornikov, D. L.
Ivanov, A. V.
Pochernikov, D. G.
Panacheva, E. A.
Issue Date: 2020
Publisher: Pirogov Russian National Research Medical University
Citation: Semen microbiota: Cluster analysis of real-time PCR data [МИКРОБИОТА ЭЯКУЛЯТА: КЛАСТЕРНЫЙ АНАЛИЗ РЕЗУЛЬТАТОВ, ПОЛУЧЕННЫХ ПРИ ИССЛЕДОВАНИИ МЕТОДОМ ПЦР-РВ] / E. S. Voroshilina, D. L. Zornikov, A. V. Ivanov, et al. — DOI 10.24075/brsmu.2020.064 // Bulletin of Russian State Medical University. — 2020. — Iss. 5. — P. 62-69.
Abstract: To this day semen microbiota is still poorly understood, and clinical significance of detecting specific microorganism groups has not been clearly determined. The aim of this work was to conduct cluster analysis of semen microbiota detected using real-time PCR. 634 semen samples of reproductive age men were analyzed using the Androflor kit. Microbial DNA in the quantity of no less than 103 GE/ml was detected in 460 samples (72.5%). From 1 to 14 microorganism groups were detected in 350 samples (55.2%) in the quantities that exceeded the threshold values (the detection rate of specific groups: 3.3–21.0%). In these 350 samples 4 stable microbiota clusters were determined. Each of the clusters was characterized by the prevalence of a specific microorganism group: obligate anaerobes (cluster 1; n = 172; detection rate — 49.1%), Lactobacillus spp. (cluster 2; n = 78; detection rate — 22.3%), gram-positive facultative anaerobes (cluster 3; n = 62; detection rate — 17.7%), Enterobacteriaceae / Enterococcus (cluster 4; n = 62; detection rate — 10.9%). Cluster 1 was less stable and was characterized by the larger species diversity compared to other clusters. © 2020 Pirogov Russian National Research Medical University. All rights reserved.
Keywords: CLUSTER ANALYSIS
REAL-TIME PCR
SEMEN ANALYSIS
SEMEN MICROBIOTA
ADULT
ANAEROBE
ARTICLE
CLUSTER ANALYSIS
ENTEROBACTERIACEAE
ENTEROCOCCUS
HUMAN
HUMAN TISSUE
LACTOBACILLUS
MAJOR CLINICAL STUDY
MALE
MICROFLORA
MICROORGANISM
NONHUMAN
PREVALENCE
REAL TIME POLYMERASE CHAIN REACTION
SEMEN ANALYSIS
SPECIES DIVERSITY
URI: http://hdl.handle.net/10995/103233
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85097641326
PURE ID: 20390239
fad1d782-e104-40ac-957c-b9214eed342d
ISSN: 25001094
DOI: 10.24075/brsmu.2020.064
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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