Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/103167
Title: A combined Langendorff-injection technique for simultaneous isolation of single cardiomyocytes from atria and ventricles of the rat heart
Authors: Butova, X. A.
Myachina, T. A.
Khokhlova, A. D.
Issue Date: 2021
Publisher: Elsevier B.V.
Citation: Butova X. A. A combined Langendorff-injection technique for simultaneous isolation of single cardiomyocytes from atria and ventricles of the rat heart / X. A. Butova, T. A. Myachina, A. D. Khokhlova. — DOI 10.1016/j.mex.2020.101189 // MethodsX. — 2021. — Vol. 8. — 101189.
Abstract: Single cardiomyocytes are widely used for investigations of the cellular and molecular mechanisms of regulation and modulation of cardiac performance. Intact cardiomyocytes allow one to study in detail cell function avoiding the effects of extracellular matrix and neighboring cells. The most established protocols of cardiomyocyte isolation are based on the isolated heart perfusion using a Langendorff-apparatus or on intraventricular perfusion using a syringe. However, the yield of single cardiomyocytes obtained by these methods may be low due to the cell injury following non-uniform enzyme digestion of connective tissue in different heart chambers. Moreover, isolation of atrial cardiomyocytes is challenging because of their small size and complex geometric shape. Here we present a new protocol for simultaneous isolation of high quality cardiomyocytes from the atria, ventricular free walls and interventricular septum. The protocol is based on the combination of the Langendorff perfusion method with the intraventricular and intra-atrial injection technique taking into account the collagen content variation between the different heart chambers. Obtained cells demonstrate rod-shaped morphology, a clear and regular sarcomere striation pattern and rat-specific frequency-dependence of contraction and calcium transient parameters. Our protocol provides gentle cell isolation that increases the yield of single cardiomyocytes suitable for biophysical researches. © 2020 The Authors
Keywords: ATRIUM
CALCIUM IMAGING
CARDIOMYOCYTE ISOLATION
INJECTION TECHNIQUE
INTERVENTRICULAR SEPTUM
LANGENDORFF-BASED PERFUSION
LANGENDORFF-INJECTION
SINGLE CELL MECHANICS
VENTRICULAR FREE WALL
CALCIUM
COLLAGEN
ADULT
ANIMAL CELL
ANIMAL EXPERIMENT
ANIMAL TISSUE
ARTICLE
CARDIAC MUSCLE CELL
CELL ISOLATION
CELL SUSPENSION
HEART ATRIUM
HEART LEFT VENTRICLE
HEART PERFUSION
HEART RIGHT VENTRICLE
HEART VENTRICLE
HEART VENTRICLE WALL
INTERVENTRICULAR SEPTUM
INTRAATRIAL DRUG ADMINISTRATION
INTRACEREBROVENTRICULAR DRUG ADMINISTRATION
ISOLATED HEART
NONHUMAN
PRIORITY JOURNAL
RAT
SARCOMERE
URI: http://hdl.handle.net/10995/103167
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85099325302
PURE ID: 20517083
ef863601-7b4b-440f-b5b4-fac4ae203968
ISSN: 22150161
DOI: 10.1016/j.mex.2020.101189
metadata.dc.description.sponsorship: Supported by President Grant of the RF #МК-949.2019.4, Russian Foundation for Basic Research # 20-315-70006, and the theme of the IIF UrB RAS #AAAA-A19-119070190064-4.
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

Files in This Item:
File Description SizeFormat 
2-s2.0-85099325302.pdf1,56 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.