Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/102728
Title: Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels
Authors: Jiang, Y.
Schiavon, M.
Lima, L. W.
Tripti
Jones, R. R.
El, Mehdawi, A. F.
Royer, S.
Zeng, Z.
Hu, Y.
Pilon-Smits, E. A. H.
Pilon, M.
Issue Date: 2018
Publisher: Elsevier B.V.
Citation: Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels / Y. Jiang, M. Schiavon, L. W. Lima, et al. — DOI 10.1016/j.bbagen.2018.03.014 // Biochimica et Biophysica Acta - General Subjects. — 2018. — Vol. 1862. — Iss. 11. — P. 2363-2371.
Abstract: Background: The plant Stanleya pinnata hyperaccumulates Se up to 0.5% of its dry weight in organic forms, whereas the closely related Stanleya elata does not hyperaccumulate Se. ATP sulfurylase (ATPS) can catalyze the formation of adenosine 5′-phosphoselenate (APSe) from ATP and selenate. We investigated the S. pinnata ATPS2 isoform (SpATPS2) to assess its possible role in Se hyperaccumulation. Methods: ATPS expression and activity was compared in the two Stanleya species. The ATPS2 protein sequences were modeled. Sub-cellular locations were analyzed using GFP fusions. Enzyme activity of purified recombinant SpATPS2 was measured. Results: ATPS2 transcript levels were six-fold higher in roots of S. pinnata relative to S. elata. Overall root ATPS enzyme activity was two-fold elevated in S. pinnata. Cloning and sequencing of SpATPS2 and S. elata ATPS2 (SeATPS2) showed the predicted SeATPS2 to be canonical, while SpATPS2, although very similar in its core structure, has unique features, including an interrupted plastid targeting signal due to a stop codon in the 5′ region of the coding sequence. Indeed GFP fusions revealed that SpATPS2 had exclusive cytosolic localization, while SeATPS2 showed dual localization in plastids and cytosol. SpATPS2 activity was inhibited by both sulfate and selenate, indicating that the enzyme acts on both substrates. Conclusions: The ATPS2 from S. pinnata differs from non-accumulator ATPS2 in its elevated expression and sub-cellular localization. It likely acts on both selente and sulfate substrates. General significance: These observations shed new light on the role of ATPS2 in the evolution of Se hyperaccumulation in plants. This article is part of a Special Issue entitled Selenium research in biochemistry and biophysics - 200 year anniversary issue, edited by Dr. Elias Arnér and Dr. Regina Brigelius-Flohe. © 2018 Elsevier B.V.
Keywords: ATP SULFURYLASE
HYPERACCUMULATION
SELENIUM
STANLEYA PINNATA
FUSION PROTEIN
GREEN FLUORESCENT PROTEIN
MESSENGER RNA
RECOMBINANT ENZYME
SELENATE
SULFATE
SULFATE ADENYLYLTRANSFERASE
SULFATE ADENYLYLTRANSFERASE 2
UNCLASSIFIED DRUG
AMINO ACID SEQUENCE
ARTICLE
BRASSICACEAE
CELLULAR DISTRIBUTION
COMPARATIVE STUDY
CONTROLLED STUDY
CYTOSOL
ENZYME ACTIVITY
ENZYME ASSAY
ENZYME STRUCTURE
MOLECULAR CLONING
NONHUMAN
PLANT ROOT
PLASMID
PLASTID
PRIORITY JOURNAL
PROTEIN EXPRESSION
PROTEIN PURIFICATION
SEQUENCE ALIGNMENT
SEQUENCE ANALYSIS
STANLEYA ELATA
STANLEYA PINNATA
STOP CODON
URI: http://hdl.handle.net/10995/102728
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85044005348
PURE ID: 7759503
2870266f-ca7f-4b0d-a3a8-8f2ac60609dd
ISSN: 3044165
DOI: 10.1016/j.bbagen.2018.03.014
Appears in Collections:Научные публикации, проиндексированные в SCOPUS и WoS CC

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