Please use this identifier to cite or link to this item: http://hdl.handle.net/10995/101476
Title: Omnidirectional microscopy by ultrasonic sample control
Authors: Helander, P.
Puranen, T.
Meriläinen, A.
Maconi, G.
Penttilä, A.
Gritsevich, M.
Kassamakov, I.
Salmi, A.
Muinonen, K.
Hæggström, E.
Issue Date: 2020
Publisher: American Institute of Physics Inc.
Citation: Omnidirectional microscopy by ultrasonic sample control / P. Helander, T. Puranen, A. Meriläinen, et al. — DOI 10.1063/5.0002602 // Applied Physics Letters. — 2020. — Vol. 116. — Iss. 19. — 194101.
Abstract: Omnidirectional microscopy (OM) is an emerging technology capable of enhancing the three-dimensional (3D) microscopy widely applied in life sciences. In OM, the precise position and orientation control of the sample are required. However, current OM technology relies on destructive mechanical methods to hold the samples, such as embedding samples in gel or attaching them to a needle to permit orientation control. A non-contacting alternative is to levitate the sample. Until now, levitation methods have lacked orientation control. We enable omnidirectional access to the sample by introducing a method to control acoustic levitation that provides precise orientation control. Such control around three axes of rotation permits rapid imaging of the sample from any direction using a fixed camera and subsequent 3D shape reconstruction. The control of non-spherical particles is achieved using an asymmetric acoustic field created with a phase-controlled transducer array. Our technology allows robust 3D imaging of delicate samples and their study in a time-lapse manner. We foresee that the described method is not limited to microscopy and optical imaging, but is also compatible with automated sample handling, light-sheet microscopy, wall-less chemistry, and non-contacting tomography. © 2020 American Institute of Physics Inc.. All rights reserved.
Keywords: PHYSICAL PROPERTIES
PHYSICS
3D SHAPE RECONSTRUCTION
ACOUSTIC LEVITATION
EMERGING TECHNOLOGIES
LIGHT-SHEET MICROSCOPIES
MECHANICAL METHODS
NONSPHERICAL PARTICLE
ORIENTATION CONTROL
THREE-DIMENSIONAL (3D) MICROSCOPY
ACOUSTIC FIELDS
URI: http://hdl.handle.net/10995/101476
Access: info:eu-repo/semantics/openAccess
SCOPUS ID: 85089088432
PURE ID: 13402690
ISSN: 36951
DOI: 10.1063/5.0002602
metadata.dc.description.sponsorship: This research was supported by European Research Council Advanced Grant No. 320773 entitled ?Scattering and Absorption of ElectroMagnetic waves in ParticuLate media? (ERC SAEMPL, 2013-2018, PI K. Muinonen). M.G. and K.M. acknowledge Academy of Finland Projects Nos. 325806 and 325805, respectively. The authors thank Joni M?akinen for his help on the use of COMSOL MultiphysicsVR simulation software. The authors have filed a patent related to the method for creating the acoustic trap.
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