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Полная запись метаданных
Поле DC | Значение | Язык |
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dc.contributor.author | Becker, N. S. | en |
dc.contributor.author | Rollins, R. E. | en |
dc.contributor.author | Nosenko, K. | en |
dc.contributor.author | Paulus, A. | en |
dc.contributor.author | Martin, S. | en |
dc.contributor.author | Krebs, S. | en |
dc.contributor.author | Takano, A. | en |
dc.contributor.author | Sato, K. | en |
dc.contributor.author | Kovalev, S. Y. | en |
dc.contributor.author | Kawabata, H. | en |
dc.contributor.author | Fingerle, V. | en |
dc.contributor.author | Margos, G. | en |
dc.date.accessioned | 2021-08-31T14:56:54Z | - |
dc.date.available | 2021-08-31T14:56:54Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | High conservation combined with high plasticity: Genomics and evolution of Borrelia bavariensis / N. S. Becker, R. E. Rollins, K. Nosenko, et al. — DOI 10.1186/s12864-020-07054-3 // BMC Genomics. — 2020. — Vol. 21. — Iss. 1. — 702. | en |
dc.identifier.issn | 14712164 | - |
dc.identifier.other | Final | 2 |
dc.identifier.other | All Open Access, Gold, Green | 3 |
dc.identifier.other | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092576728&doi=10.1186%2fs12864-020-07054-3&partnerID=40&md5=e1e1d32799a064019843ea635af95061 | |
dc.identifier.other | https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-020-07054-3 | m |
dc.identifier.uri | http://elar.urfu.ru/handle/10995/101391 | - |
dc.description.abstract | Background: Borrelia bavariensis is one of the agents of Lyme Borreliosis (or Lyme disease) in Eurasia. The genome of the Borrelia burgdorferi sensu lato species complex, that includes B. bavariensis, is known to be very complex and fragmented making the assembly of whole genomes with next-generation sequencing data a challenge. Results: We present a genome reconstruction for 33 B. bavariensis isolates from Eurasia based on long-read (Pacific Bioscience, for three isolates) and short-read (Illumina) data. We show that the combination of both sequencing techniques allows proper genome reconstruction of all plasmids in most cases but use of a very close reference is necessary when only short-read sequencing data is available. B. bavariensis genomes combine a high degree of genetic conservation with high plasticity: all isolates share the main chromosome and five plasmids, but the repertoire of other plasmids is highly variable. In addition to plasmid losses and gains through horizontal transfer, we also observe several fusions between plasmids. Although European isolates of B. bavariensis have little diversity in genome content, there is some geographic structure to this variation. In contrast, each Asian isolate has a unique plasmid repertoire and we observe no geographically based differences between Japanese and Russian isolates. Comparing the genomes of Asian and European populations of B. bavariensis suggests that some genes which are markedly different between the two populations may be good candidates for adaptation to the tick vector, (Ixodes ricinus in Europe and I. persulcatus in Asia). Conclusions: We present the characterization of genomes of a large sample of B. bavariensis isolates and show that their plasmid content is highly variable. This study opens the way for genomic studies seeking to understand host and vector adaptation as well as human pathogenicity in Eurasian Lyme Borreliosis agents. © 2020 The Author(s). | en |
dc.description.sponsorship | Robert-Koch-Institut funded strain isolation, cultivation and Illumina sequencing for 33 isolates at the NRZ Borrelia. PacBio sequencing for tree isolates was financed by the ESCMID Study Group for Lyme Borreliosis. qPCR experiments were funded through the German Research Foundation (DFG Grant No. BE 5791/2-1). Open Access funding enabled and organized by Projekt DEAL. | en |
dc.format.mimetype | application/pdf | en |
dc.language.iso | en | en |
dc.publisher | BioMed Central Ltd | en |
dc.rights | info:eu-repo/semantics/openAccess | en |
dc.source | BMC Genomics | 2 |
dc.source | BMC Genomics | en |
dc.subject | BORRELIA BAVARIENSIS | en |
dc.subject | GENETIC PLASTICITY | en |
dc.subject | GENOME ASSEMBLY | en |
dc.subject | LYME BORRELIOSIS | en |
dc.subject | PLASMIDS | en |
dc.subject | ARTICLE | en |
dc.subject | ASIAN | en |
dc.subject | BACTERIAL CHROMOSOME | en |
dc.subject | BACTERIAL GENETICS | en |
dc.subject | BACTERIAL VIRULENCE | en |
dc.subject | BACTERIUM ISOLATE | en |
dc.subject | BORRELIA | en |
dc.subject | BORRELIA BAVARIENSIS | en |
dc.subject | EUROPE | en |
dc.subject | GENE FUSION | en |
dc.subject | GENE SEQUENCE | en |
dc.subject | GENE TRANSFER | en |
dc.subject | GENETIC CONSERVATION | en |
dc.subject | GENETIC VARIABILITY | en |
dc.subject | HOST MICROBE INTERACTION | en |
dc.subject | HUMAN | en |
dc.subject | IXODES PERSULCATUS | en |
dc.subject | IXODES RICINUS | en |
dc.subject | JAPANESE (PEOPLE) | en |
dc.subject | LYME DISEASE | en |
dc.subject | NONHUMAN | en |
dc.subject | PLASMID | en |
dc.subject | RUSSIAN (CITIZEN) | en |
dc.subject | ANIMAL | en |
dc.subject | ASIA | en |
dc.subject | BACTERIAL GENOME | en |
dc.subject | BORRELIELLA | en |
dc.subject | CLASSIFICATION | en |
dc.subject | CONSERVED SEQUENCE | en |
dc.subject | GENETICS | en |
dc.subject | GENOMICS | en |
dc.subject | IXODES | en |
dc.subject | LYME DISEASE | en |
dc.subject | MICROBIOLOGY | en |
dc.subject | PHYLOGENY | en |
dc.subject | RUSSIAN FEDERATION | en |
dc.subject | SPIROCHAETALES | en |
dc.subject | ANIMALS | en |
dc.subject | ASIA | en |
dc.subject | BORRELIA BURGDORFERI GROUP | en |
dc.subject | CONSERVED SEQUENCE | en |
dc.subject | EUROPE | en |
dc.subject | GENOME, BACTERIAL | en |
dc.subject | GENOMICS | en |
dc.subject | HUMANS | en |
dc.subject | IXODES | en |
dc.subject | LYME DISEASE | en |
dc.subject | PHYLOGENY | en |
dc.subject | PLASMIDS | en |
dc.subject | RUSSIA | en |
dc.subject | SPIROCHAETALES | en |
dc.title | High conservation combined with high plasticity: Genomics and evolution of Borrelia bavariensis | en |
dc.type | Article | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | info:eu-repo/semantics/publishedVersion | en |
dc.identifier.doi | 10.1186/s12864-020-07054-3 | - |
dc.identifier.scopus | 85092576728 | - |
local.contributor.employee | Becker, N.S., Division of Evolutionary Biology, Faculty of Biology, Lmu Munich, Grosshaderner Strasse 2, Planegg-Martinsried, 82152, Germany | |
local.contributor.employee | Rollins, R.E., Division of Evolutionary Biology, Faculty of Biology, Lmu Munich, Grosshaderner Strasse 2, Planegg-Martinsried, 82152, Germany | |
local.contributor.employee | Nosenko, K., Division of Evolutionary Biology, Faculty of Biology, Lmu Munich, Grosshaderner Strasse 2, Planegg-Martinsried, 82152, Germany | |
local.contributor.employee | Paulus, A., Division of Evolutionary Biology, Faculty of Biology, Lmu Munich, Grosshaderner Strasse 2, Planegg-Martinsried, 82152, Germany | |
local.contributor.employee | Martin, S., Division of Evolutionary Biology, Faculty of Biology, Lmu Munich, Grosshaderner Strasse 2, Planegg-Martinsried, 82152, Germany, University of Helsinki, Biomedicum Helsinki, Haartmaninkatu 8, PO Box 63, Helsinki, FIN-00014, Finland | |
local.contributor.employee | Krebs, S., Gene Center, Laboratory for Functional Genome Analysis, Lmu Munich, Feodor-Lynen-Strasse 25, Munich, 81377, Germany | |
local.contributor.employee | Takano, A., Department of Veterinary Epidemiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8515, Japan | |
local.contributor.employee | Sato, K., Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan | |
local.contributor.employee | Kovalev, S.Y., Laboratory of Molecular Genetics, Institute of Natural Sciences and Mathematics, Ural Federal University, Lenin Avenue 51, Yekaterinburg, 620000, Russian Federation | |
local.contributor.employee | Kawabata, H., Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan | |
local.contributor.employee | Fingerle, V., National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority, Veterinärstr 2, Oberschleissheim, 85764, Germany | |
local.contributor.employee | Margos, G., National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority, Veterinärstr 2, Oberschleissheim, 85764, Germany | |
local.issue | 1 | - |
local.volume | 21 | - |
dc.identifier.wos | 000578575900002 | - |
local.contributor.department | Division of Evolutionary Biology, Faculty of Biology, Lmu Munich, Grosshaderner Strasse 2, Planegg-Martinsried, 82152, Germany | |
local.contributor.department | University of Helsinki, Biomedicum Helsinki, Haartmaninkatu 8, PO Box 63, Helsinki, FIN-00014, Finland | |
local.contributor.department | Gene Center, Laboratory for Functional Genome Analysis, Lmu Munich, Feodor-Lynen-Strasse 25, Munich, 81377, Germany | |
local.contributor.department | Department of Veterinary Epidemiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8515, Japan | |
local.contributor.department | Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan | |
local.contributor.department | Laboratory of Molecular Genetics, Institute of Natural Sciences and Mathematics, Ural Federal University, Lenin Avenue 51, Yekaterinburg, 620000, Russian Federation | |
local.contributor.department | National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority, Veterinärstr 2, Oberschleissheim, 85764, Germany | |
local.identifier.pure | 37f8defb-32d5-4c36-b247-468706d9af4d | uuid |
local.identifier.pure | 14159644 | - |
local.description.order | 702 | - |
local.identifier.eid | 2-s2.0-85092576728 | - |
local.identifier.wos | WOS:000578575900002 | - |
local.identifier.pmid | 33032522 | - |
Располагается в коллекциях: | Научные публикации ученых УрФУ, проиндексированные в SCOPUS и WoS CC |
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2-s2.0-85092576728.pdf | 1,31 MB | Adobe PDF | Просмотреть/Открыть |
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