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dc.contributor.authorTrofimov, A. G.en
dc.contributor.authorVershinina, S. D.en
dc.date.accessioned2018-11-17T15:18:17Z-
dc.date.available2018-11-17T15:18:17Z-
dc.date.issued2018-
dc.identifier.citationTrofimov A. G. About the Applicability of Bone and Cartilage Acid-free Staining for Anura / A. G. Trofimov, S. D. Vershinina // The Second International Conference on Amphibian and Reptiles Anomalies and Pathology (Ekaterinburg, Russia, 06–10 September, 2016). – Dubai : Knowledge E, 2018. – pp. 156-160. – DOI: 10.18502/kls.v4i3.2118en
dc.identifier.issn2413-0877-
dc.identifier.urihttp://elar.urfu.ru/handle/10995/64859-
dc.description.abstractThe article describes the first use of acid-free alcian-alizarin staining for tailless amphibians in comparison with the standard method of acid staining on the example of the moor frog. The acid-free protocol is applicable for Anura and it has a number of advantages, such as the absence of decalcification, safety for the researcher and others. However, this method also has limitations, such as the binding of alcian blue with soft tissues.en
dc.description.sponsorshipThe authors thank Dmitriy Berzin and Natalia Ivanova for their help with growing tadpoles, Maxim Kovrizhin and Julia Sanaeva for their help with the chemical calculations, Yulia Kruzhkova for advice on the acid method of staining, Artem A. Minin, Artem S. Minin, Victor Sapronov, Ivan Sitnikov and Olga Zagainova for their help with the equipment and reagents and Vladimir Vershinin for his helpful comments and moral support.en
dc.description.sponsorshipThe work was performed within the frameworks of state contract with the Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, and partly supported by complex program of UBRAS (project № 18-4-4-28) and by Act 211, Government of the Russian Federation, contract № 02.A03.21.0006.en
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherKnowledge Een
dc.relation.ispartofThe Second International Conference on Amphibian and Reptiles Anomalies and Pathology. — Ekaterinburg, 2016en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subjectANURAen
dc.subjectFROGSen
dc.subjectRANA ARVALISen
dc.subjectDEVELOPMENTen
dc.subjectWHOLE-MOUNT PREPARATIONen
dc.subjectDOUBLE STAININGen
dc.subjectALCIAN BLUEen
dc.subjectALIZARIN REDen
dc.subjectBONEen
dc.subjectCARTILAGEen
dc.subjectDECALCIFICATIONen
dc.titleAbout the Applicability of Bone and Cartilage Acid-free Staining for Anuraen
dc.typeConference Paperen
dc.typeinfo:eu-repo/semantics/conferenceObjecten
dc.typeinfo:eu-repo/semantics/publishedVersionen
dc.conference.nameThe Second International Conference on Amphibian and Reptiles Anomalies and Pathologyen
dc.conference.date06.09.2016-10.09.2016-
dc.identifier.doi10.18502/kls.v4i3.2118-
dc.rights.licenseCreative Commons Attribution Licenseen
local.description.firstpage156-
local.description.lastpage160-
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