Исследование грибных сообществ, ассоциированных с древесным детритом, методом ДНК-метабаркодинга

Abstract

To study fungal communities associated with wood debris the modern DNA metabarcoding method was implemented. With specified method 17 samples of Pinus sylvestris, Picea abies and Betula sp. wood debris were analyzed, and 61536 nucleotide sequences of ITS2 region for further bioinformatics processing were received. Basing on ITS2 region polymorphism 47 operational taxonomical units (OTU’s) were determined. The majority of them were identified to species level. The following species and genera represent dominant fungi, which were found in different samples: Fomes fomentarius, Fomitopsis pinicola, Ganoderma applanatum, Ischnoderma benzoinum, Phellinus chrysoloma, Phlebiopsis gigantea, Rhodonia placenta, Piptoporus betulinus, Steccherinum tenuispinum, Nodulisporium sp., Phialocephala sp., Porodaedalia pini, Hypocrea virens, Leptodontidium sp. The number of ITS2 region copies of these fungi significantly exceeded number of copies of other OTU’s in the considered samples. In certain cases, different dominant species revealed in one sample simultaneously. As minor component we found Anomoloma albolutescens, Austrolecia sp., Blastobotrys proliferans, Cladonia pyxidata, Cladosporium sp., Hyphoderma puberum, Hyphoderma setigerum, Hyphoderma subtestaceum, Lecythophora sp., Penicillium toxicarium, Oidiodendron griseum, Rhodotorula philyla, Rhodotorula pustula, Rhinocladiella sp., Sistotrema brinkmannii, Spathaspora arborariae, Sugiyamaella sp., Trichoderma harzianum, Trametes versicolor.