Экофизиологические особенности редкого вида Sparassis crispa (Sparassidaceae, Basidiomycota) в условиях чистой культуры

Abstract

Growth of two strains of Sparassis crispa s str. (LE-BIN 043 and 2902) under various temperatures and substrate’s acidity was studied to give insight into ecological characteristics of this rare species. The general patterns of mean growth rate with respect to these abiotic factors were similar for both studied isolates. Temperature optimum was 20–25 °C. Mean growth rate declined sharply at temperatures below 20 °C and no growth was detected at temperatures 30 and 35 °C. Growth of both strains was renewed after the transference of Petri dishes at 20 °C but a lag phase was longer than in control. There was no growth after exposure at –20 °C for 2 weeks with the exception for 1 replication of strain 043. But lag of this colony was longer than after 35 °C and mean growth rate was less than in control. Both strains grew similarly at pH 3.55–6.05 however growth was absent at pH 6.55. After 1.5 month of growth рН of the medium was 2.8–3.5 despite of initial acidity. Therefore, S. crispa may be considered as a rather stenoecic species with regard to temperature and substrate acidity.