ДНК-анализ отцовства у стерхов (Grus leucogeranus pallas) при искусственном осеменении

Abstract

Using 10 polymorphic microsatellite loci (Gpa-12, Gpa-32, Gpa-38, Gpa-39, Gram-22, Gram-30, Gj-M15, Gj-M34, Gj-M8, Gj-2298), paternity of 64 individuals of the Siberian crane obtained under multiple artificial insemination has been analyzed. DNA-analysis revealed 30 and 34 cases of correct and wrong paternities, correspondently. Paternities of 28 birds belonged to the sires whose spermatozoa kept survival into the dam’s reproductive ducts during 4–17 days. In 6 cases paternities were established for imprinted and injured breeding partners of the females.