Особенности влияния некоторых углеводсодержащих сред на процесс формирования биопленок бактерией Pectobacterium carotovorum

Abstract

The aim of our study was to study the effect of carbon sources such as setiathome alcohols (dulcic, Inositol, mannitol), sugars (D-xylose, lactose, maltose and fructose) and amino acid (tryptophan), on the formation and development of biofilms by phytopathogenic bacterium P.carotovorum in laboratory conditions. To study the effect of carbon sources in the environment on the dynamics of the formation of biofilms P.carotovorum used method of light microscopy and spectrophotometric studies. Used in the experiment of compounds the most favorable substrate for the formation of biofilms has been Inositol, which contributed to the formation of multicellular communities (1 day – 365 ± 273 cells, 6 day – 915 ± 176 cells, 8 day – 3529 ± 586 cells). Medium without carbon source (buffered saline), the least favorable for the cultivation of bacteria (on the 1st day of 19 ± 11 cells). In environments with the introduction of the carbon, especially with the addition of Inositol, bacterial cells were more elongated shape, as evidenced by the ratio of length to width (glucose – 2.4; Inositol – 3,5). The most favorable was the medium with the content of the polyhydric alcohol Inositol, in the medium with the content of the carbon source bacteria more intensively formed biofilms.