Подходы к изучению механизмов антигрибного действия харпино-подобных защитных пептидов растений

Abstract

An interaction of hairpin-like cationic peptide EcAMP1 from barnyard grass (Echinochloa crusgalli L.) with conidia of plant pathogenic fungus Fusarium solani at the cellular level was studied by combination of optical, laser scanning fluorescence and atomic force microscopy. As result, a direct relationship between hyphal growth inhibition and increasing of peptide active concentration, time of incubation and fungal physiological condition has been determined. A dynamics of accumulation and redistribution of the peptide studied on fungal cellular cover and inside the conidia cells depending on time of coupling has been shown, also a dissimilarity of EcAMP1 binding with fungal cover, and its stepwise accumulation and diffuse localization in cytoplasm. A correlation between structural disruption of fungal conidia under influence of peptide high concentrations and presence of morphological changes at concentration above 32 µM has been also found.