Развитие женского гаметофита у трансгенных растений с экспрессией гетерологичного гена hMG1

Abstract

Isoprenoids are the largest class of plant metabolites involved in growth, plant development and the formation of generative organs. The biosynthesis of these compounds in cytoplasm is limited by the formation of mevalonic acid, which is coded by the hmg1 gene. Usage of the strategy of antisense RNA can inhibit the formation of mevalonate and reduce the biosyn­thesis of isoprenoids in plants. Transgenic tobacco plants expressing the hmg1 gene in reverse orientation towards the constitutive viral promoter CaMV 35S were obtained. The investigation of ovules morphology in transgenic tobacco lines showed that abnormal ovules occurred 1.5-fold frequently in comparison with control plants. Thus, there are multiple pathologies in the development of both male and female generative sphere under suppression of the mevalonic acid biosynthesis in plants.