ПЦР-анализ аллельного состояния Wx-локусов в генотипах селекционных форм мягкой пшеницы

Abstract

The aim of work was to reveal the alleles of Wx-loci by PCR method in 16 breading forms of bread wheat that have been selected in Yurjev Plant Production Institute (Kharkov). We have detected the presence of null­allele Wx-A1b in genotypes 146-02/5, 146-07/1, 146-07/2; Wx-B1b allele — in genotype 146-06/3 and both null-alleles (Wx-A1b and Wx-B1b) in genotypes of 146-02/1, 146-02/2; in the other lines the alleles Wx-A1a, Wx-B1a, Wx-D1a have been tested.. Any form did not contain Wx-D1b allele. Forms with null­alleles will be used for creation of wheat variety with low content of amylose in endosperm of grains.