Экспрессия пептида М2e вируса гриппа птиц H5N1 в трансгенных растениях ряски (Lemna minor L.)

Abstract

N-terminal segment of M2 protein 30 amino acids in length, including peptide M2e was fused to the N- terminus of в-glucuronidase or C-terminus of ricin subunit B in the plant expression vector pBI121. The nucleotide sequence of the target fragment was synthesized by ligation of the synthetic oligonucleotides, its codon usage have been adapted previously for plant expression. As a result of peptide M2e of avian influenza virus H5N1Cur­gan 2005 was successfully expressed in transgenic duckweed plants.