ISSN: 2310-757X
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http://elar.urfu.ru/handle/10995/64859| Название: | About the Applicability of Bone and Cartilage Acid-free Staining for Anura |
| Авторы: | Trofimov, A. G. Vershinina, S. D. |
| Дата публикации: | 2018 |
| Издатель: | Knowledge E |
| Библиографическое описание: | Trofimov A. G. About the Applicability of Bone and Cartilage Acid-free Staining for Anura / A. G. Trofimov, S. D. Vershinina // The Second International Conference on Amphibian and Reptiles Anomalies and Pathology (Ekaterinburg, Russia, 06–10 September, 2016). – Dubai : Knowledge E, 2018. – pp. 156-160. – DOI: 10.18502/kls.v4i3.2118 |
| Аннотация: | The article describes the first use of acid-free alcian-alizarin staining for tailless amphibians in comparison with the standard method of acid staining on the example of the moor frog. The acid-free protocol is applicable for Anura and it has a number of advantages, such as the absence of decalcification, safety for the researcher and others. However, this method also has limitations, such as the binding of alcian blue with soft tissues. |
| Ключевые слова: | ANURA FROGS RANA ARVALIS DEVELOPMENT WHOLE-MOUNT PREPARATION DOUBLE STAINING ALCIAN BLUE ALIZARIN RED BONE CARTILAGE DECALCIFICATION |
| URI: | http://elar.urfu.ru/handle/10995/64859 |
| Текст лицензии: | https://creativecommons.org/licenses/by/4.0/ |
| Конференция/семинар: | The Second International Conference on Amphibian and Reptiles Anomalies and Pathology |
| Дата конференции/семинара: | 06.09.2016-10.09.2016 |
| ISSN: | 2413-0877 |
| DOI: | 10.18502/kls.v4i3.2118 |
| Сведения о поддержке: | The authors thank Dmitriy Berzin and Natalia Ivanova for their help with growing tadpoles, Maxim Kovrizhin and Julia Sanaeva for their help with the chemical calculations, Yulia Kruzhkova for advice on the acid method of staining, Artem A. Minin, Artem S. Minin, Victor Sapronov, Ivan Sitnikov and Olga Zagainova for their help with the equipment and reagents and Vladimir Vershinin for his helpful comments and moral support. The work was performed within the frameworks of state contract with the Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, and partly supported by complex program of UBRAS (project № 18-4-4-28) and by Act 211, Government of the Russian Federation, contract № 02.A03.21.0006. |
| Источники: | The Second International Conference on Amphibian and Reptiles Anomalies and Pathology. — Ekaterinburg, 2016 |
| Располагается в коллекциях: | Конференции, семинары |
Файлы этого ресурса:
| Файл | Описание | Размер | Формат | |
|---|---|---|---|---|
| kls.v4i3.2118.pdf | 362,94 kB | Adobe PDF | Просмотреть/Открыть |
Лицензия на ресурс: Лицензия Creative Commons
